Higenamine (HG) is a β2 receptor agonist and was explicitly added to the Prohibited List of the World Anti‐Doping Agency in 2017. This compound is prohibited in both in‐ and out‐of‐competition athletes and falls under the category of nonthreshold substances. Because of HG presence in numerous plants, as evidenced by a growing body of research data, an exception was made for HG in the TD2017MRPL document, in which adverse analytical findings (AAFs) were not reported if the urinary HG concentration was less than 10 ng/mL. In this study, a comprehensive and systematic analysis of the HG content in five batches of samples from each of the 48 natural spices selected for this investigation was conducted using UPLC–MS/MS technology. Method validation was carried out in accordance with the ICH Analytical Procedures and Methods Validation for Drugs and Biologics Guidance, and the experimental results demonstrated that the method provided appropriate sensitivity, precision, stability, linearity, and accuracy. HG was detected for the first time in Houttuynia cordata, Zingiber officinale, Cinnamomum cassia, Stevia rebaudiana, Piper nigrum, Siraitia grosuenorii, Platycodon grandiflorus, and Myristica fragrans. Furthermore, the content of HG was found to vary significantly among the different plant parts of Nelumbo nucifera, such as rhizomes, leaves, seeds, and plumules. This paper provides systematic and comprehensive data to support the safe use of spices in athletes' diets, thereby reducing the risk of food‐sourced doping violations.

中文翻译：

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超高效液相色谱-串联质谱法测定常见天然香料中去甲乌药碱的含量

去甲乌药碱 (HG) 是一种 β2受体激动剂，并于 2017 年被明确列入世界反兴奋剂机构禁用清单。该化合物在赛内和赛外运动员中均被禁用​​，属于非阈值物质类别。正如越来越多的研究数据所证明的那样，由于许多植物中都存在 HG，因此在TD2017MRPL文件中，如果尿 HG 浓度低于 10 ng/mL，则不会报告不良分析结果 (AAF)。本研究采用 UPLC-MS/MS 技术对本次调查选取的 48 种天然香料的 5 批样品中的 HG 含量进行了全面、系统的分析。方法验证按照ICH分析程序和药物和生物制品方法验证指导原则进行，实验结果表明该方法具有适当的灵敏度、精密度、稳定性、线性和准确度。首次检测到HG鱼腥草,生姜,肉桂,甜叶菊,黑胡椒,格罗苏诺花,桔梗， 和肉豆蔻。此外，我们还发现，不同植物部位的 HG 含量存在显着差异。荷花，如根茎、叶、种子和胚芽。本文提供了系统、全面的数据来支持运动员饮食中香料的安全使用，从而降低食品源兴奋剂违规的风险。