Entomopathogenic fungi are promising biocontrol agents for pest management, but they could be sensitive to application methods. Sprayers, used for both fungal and chemical products, can potentially harm the fungal propagules. This study investigated the impact of common hydraulic pumps (piston and diaphragm) on the viability of Vuillemin (Ascomycota: Hyprocreales) based mycoinsecticide Botanigard® in the spray mixture. It also aimed to determine the optimal filter mesh size for the system to prevent clogging during application. The results showed significant reduction in conidial germination percentages with increasing number of passes through both types of pumps. However, in both cases the decrease was around 4% with a 90% of conidial germination which may be high enough to control the pest. The temperature of the mixture increased with the number of passes through the pumps, but it remained within the suitable range for fungal growth. When it came to filter clogging, the 32-mesh filter did not clog, the 50-mesh had a low clogging percentage, and the 80-mesh filters had higher clogging percentages (18% for suction filter and 56% for impulsion filter) after 30 passes through the system. As a conclusion, hydraulic pumps are suitable to apply based mycoinsecticide Botanigard®, but temperature control should be taken into account to prevent conidia damage. Using 32-mesh filters for both suction and impulsion is recommended to prevent clogging issues when applying this product.

中文翻译：

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喷雾器的液压泵和过滤器是否会影响白僵菌杀真菌剂 Botanigard® 的活性？

昆虫病原真菌是用于害虫管理的有前途的生物防治剂，但它们可能对应用方法敏感。用于真菌和化学产品的喷雾器可能会损害真菌繁殖体。本研究调查了常见液压泵（活塞和隔膜）对喷雾混合物中基于 Vuillemin（子囊菌亚门：Hyprocreales）的杀真菌剂 Botanigard® 活力的影响。它还旨在确定系统的最佳过滤网尺寸，以防止应用过程中发生堵塞。结果表明，随着通过两种类型泵的次数增加，分生孢子萌发百分比显着降低。然而，在这两种情况下，下降幅度约为 4%，分生孢子萌发率为 90%，这可能足以控制害虫。混合物的温度随着通过泵的次数而增加，但仍保持在适合真菌生长的范围内。过滤器堵塞情况方面，32目过滤器不堵塞，50目过滤器堵塞率较低，80目过滤器堵塞率较高（吸滤器为18%，脉冲过滤器为56%）。 30次通过系统。综上所述，液压泵适合使用基于杀真菌剂的Botanigard®，但应考虑温度控制以防止分生孢子损伤。建议使用 32 目过滤器进行抽吸和推动，以防止使用本产品时出现堵塞问题。