Glyphosate is an endocrine disruptor and can act on the activity of certain enzymes of metabolism subsequently altering some functions such as reproduction. The goal of the present study is to evaluate the involvement of glyphosate based-herbicide (GBH) in spermatogenesis disruption and to investigate which cells of the adult Wistar rat testis are most affected by short-term exposure to GBH. Treated groups received a diluted solution of GBH orally for 21 days (D1: 102.5 mg/Kg; D2: 200 mg/Kg; D3: 400 mg/Kg). The control group (C) received water in the same manner. Hormone levels, oxidative stress markers were evaluated, histological and morphometric analysis were performed, AR and p53 expression was conducted. Seminiferious epithelium sloughing associated to erosion of Sertoli and spermatogonia from the basement of the seminiferous tubules, with intraluminal exfoliated cells among with immature spermatids were observed. A significant change in morphometric measurement and significant decrease in AR expression in Sertoli cells were noted for all treated groups. A significant increase in NO level and p53 expression in Leydig cells were showed for animals treated with 200 and 400 mg/kg BW/day. These data demonstrate that short-term exposure to high doses of GBH has led to a disruption of certain parameters that could disturb spermatogenesis. The treatment showed that both Leydig and Sertoli cells are affected in the same manner by GBH, the activation of p53 expression in both Leydig cells and peritubular myloid cells nuclei, and the reduction in AR expression in Sertoli cells, which resulted in important testicular damage.

中文翻译：

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短期接触高剂量草甘膦除草剂后睾丸成年 Wistar 大鼠精子发生的破坏：组织病理学和生化变化

草甘膦是一种内分泌干扰物，可以作用于某些代谢酶的活性，从而改变某些功能，例如生殖。本研究的目的是评估草甘膦除草剂 (GBH) 对精子发生破坏的影响，并调查成年 Wistar 大鼠睾丸的哪些细胞受短期接触 GBH 的影响最大。治疗组口服GBH稀释溶液21天（D1：102.5 mg/Kg；D2：200 mg/Kg；D3：400 mg/Kg）。对照组（C）以相同的方式接受水。评估激素水平、氧化应激标志物，进行组织学和形态测量分析，并进行 AR 和 p53 表达。观察到与生精小管基底的支持细胞和精原细胞的侵蚀相关的生精上皮脱落，以及管腔内脱落的细胞和未成熟的精子细胞。所有治疗组均注意到支持细胞形态测量的显着变化和 AR 表达的显着降低。对于每天接受 200 和 400 mg/kg BW 处理的动物，Leydig 细胞中 NO 水平和 p53 表达显着增加。这些数据表明，短期暴露于高剂量的 GBH 会导致某些参数的破坏，从而扰乱精子发生。治疗表明，GBH 以相同的方式影响 Leydig 细胞和 Sertoli 细胞，激活 Leydig 细胞和管周淀粉样细胞核中的 p53 表达，并减少 Sertoli 细胞中的 AR 表达，从而导致重要的睾丸损伤。