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Methyl rosmarinate is an allosteric inhibitor of SARS-CoV-2 3 CL protease as a potential candidate against SARS-cov-2 infection
Antiviral Research ( IF 7.6 ) Pub Date : 2024-02-24 , DOI: 10.1016/j.antiviral.2024.105841 Hongtao Li , Meng Sun , Fuzhi Lei , Jinfeng Liu , Xixiang Chen , Yaqi Li , Ying Wang , Jiani Lu , Danmei Yu , Yueqiu Gao , Jianrong Xu , Hongzhuan Chen , Man Li , Zhigang Yi , Xiao He , Lili Chen
Antiviral Research ( IF 7.6 ) Pub Date : 2024-02-24 , DOI: 10.1016/j.antiviral.2024.105841 Hongtao Li , Meng Sun , Fuzhi Lei , Jinfeng Liu , Xixiang Chen , Yaqi Li , Ying Wang , Jiani Lu , Danmei Yu , Yueqiu Gao , Jianrong Xu , Hongzhuan Chen , Man Li , Zhigang Yi , Xiao He , Lili Chen
The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been ongoing for more than three years and urgently needs to be addressed. Traditional Chinese medicine (TCM) prescriptions have played an important role in the clinical treatment of patients with COVID-19 in China. However, it is difficult to uncover the potential molecular mechanisms of the active ingredients in these TCM prescriptions. In this paper, we developed a new approach by integrating the experimental assay, virtual screening, and the experimental verification, exploring the rapid discovery of active ingredients from TCM prescriptions. To achieve this goal, 4 TCM prescriptions in clinical use for different indications were selected to find the antiviral active ingredients in TCMs. The 3-chymotrypsin-like protease (3CL), an important target for fighting COVID-19, was utilized to determine the inhibitory activity of the TCM prescriptions and single herb. It was found that 10 single herbs had better inhibitory activity than other herbs by using a fluorescence resonance energy transfer (FRET) - based enzymatic assay of SARS-CoV-2 3CL. The ingredients contained in 10 herbs were thus virtually screened and the predicted active ingredients were experimentally validated. Thus, such a research strategy firstly removed many single herbs with no inhibitory activity against SARS-CoV-2 3CL at the very beginning by FRET-based assay, making our subsequent virtual screening more effective. Finally, 4 active components were found to have stronger inhibitory effects on SARS-CoV-2 3CL, and their inhibitory mechanism was subsequently investigated. Among of them, methyl rosmarinate as an allosteric inhibitor of SARS-CoV-2 3CL was confirmed and its ability to inhibit viral replication was demonstrated by the SARS-CoV-2 replicon system. To validate the binding mode via docking, the mutation experiment, circular dichroism (CD), enzymatic inhibition and surface plasmon resonance (SPR) assay were performed, demonstrating that methyl rosmarinate bound to the allosteric site of SARS-CoV-2 3CL. In conclusion, this paper provides the new ideas for the rapid discovery of active ingredients in TCM prescriptions based on a specific target, and methyl rosmarinate has the potential to be developed as an antiviral therapeutic candidate against SARS-CoV-2 infection.
更新日期：2024-02-24
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