A hydrogel-based wound dressing with desirable properties is necessary for achieving functional skin integrity post-injury. This study focuses on preparing a hydrogel using Alginate/Carboxymethyl cellulose (Alg/CMC) as a base material. To evaluate its regenerative effects on full-thickness wounds, diopside nanoparticles and Botulinum toxin A (BTX-A) were incorporated into the hydrogel along with chorion membrane. The diopside nanoparticles (DNPs) act as a proangiogenic factor, promoting proliferation and regulating inflammation, while the chorion membrane facilitates these processes. Additionally, BTX-A prevents scar formation and aids in wound closure. The nanoparticles and hydrogel were characterized using various techniques, and their cytocompatibility was assessed. In vivo studies and quantitative polymerase chain reaction analysis showed that wound area reduction was significant after two weeks of treatment with the Alg/CMC/ChNPs/DNPs/BTX-A hydrogel. Overall, this scaffold demonstrated potential for promoting tissue regeneration and new epithelization formation, making it a promising candidate for enhancing skin restoration in wound treatments.

具有理想特性的基于水凝胶的伤口敷料对于实现受伤后功能性皮肤完整性是必要的。本研究的重点是使用海藻酸盐/羧甲基纤维素（Alg/CMC）作为基础材料制备水凝胶。为了评估其对全层伤口的再生效果，透辉石纳米粒子和肉毒杆菌毒素 A (BTX-A) 与绒毛膜一起被纳入水凝胶中。透辉石纳米粒子（DNP）充当促血管生成因子，促进增殖和调节炎症，而绒毛膜则促进这些过程。此外，BTX-A 可防止疤痕形成并有助于伤口闭合。使用各种技术对纳米颗粒和水凝胶进行表征，并评估它们的细胞相容性。体内研究和定量聚合酶链反应分析表明，使用 Alg/CMC/ChNPs/DNPs/BTX-A 水凝胶治疗两周后，伤口面积显着减少。总体而言，该支架表现出促进组织再生和新上皮形成的潜力，使其成为伤口治疗中增强皮肤修复的有希望的候选者。