An integrated and projected-based laboratory course was described, integrating interconnected knowledge points and biochemistry and molecular biology techniques on a research project-based system. The program, which served as an essential extension of theoretical courses to practice, was conducted with a sophomore of basic medical science who had completed the course in medical biochemistry and molecular biology. This course engaged students in learning “genetic manipulation” and “recombinant DNA technology” to understand the target gene's role in disease mechanics, thus altering evaluation and treatment for clinical disease. Students could master applied and advanced techniques, such as cell culture, transfection, inducing exogenous fusion protein expression, purifying protein and its concentration assay, quantitative polymerase chain reaction, and western bot analysis. This laboratory exercise links laboratory practices with the methods of current basic research. Students need to complete the experimental design report and laboratory report, which could be advantageous for improving their ability to write lab summaries and scientific papers in the future. The reliability and validity analyses were conducted on the questionnaire, and we examined students' satisfaction with the course and their gains from the course. The student feedback was generally positive, indicating that the exercise helped consolidate theoretical knowledge, increase scientific research enthusiasm, and provide a powerful tool to be a better person and make informed decisions.

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开发基于项目的高级生物化学和分子生物学综合实验室课程


描述了基于项目的综合实验室课程，将相互关联的知识点以及生物化学和分子生物学技术集成在基于研究项目的系统上。该项目是理论课程向实践的重要延伸，由一名完成医学生物化学和分子生物学课程的基础医学二年级学生进行。本课程让学生学习“基因操作”和“重组DNA技术”，了解靶基因在疾病机制中的作用，从而改变临床疾病的评估和治疗。学生可以掌握细胞培养、转染、诱导外源融合蛋白表达、纯化蛋白及其浓度测定、定量聚合酶链反应、Western bot分析等应用和先进技术。该实验室练习将实验室实践与当前基础研究的方法联系起来。学生需要完成实验设计报告和实验报告，这有利于提高今后撰写实验总结和科技论文的能力。对问卷进行信度和效度分析，考察学生对课程的满意度和课程收获。学生反馈普遍积极，表明此次练习有助于巩固理论知识，提高科研热情，为成为更好的人、做出明智的决策提供了有力的工具。