Determining the minimum postmortem interval (minPMI) from an entomological perspective relies mainly on development data recorded for various species of flies collected from a crime scene or suspicious death. This study focused on the larval and pupal development of Lucilia sericata (Meigen), with an emphasis on the changes of the external morphology of the puparium and its pupal content throughout the duration of metamorphosis. Colonies of L. sericata were reared on 3 types of swine tissue (skeletal muscle, liver tissue, and heart tissue) at 2 different temperature regimes; 24 ± 1 °C and 30 ± 1 °C. The overall developmental time, larval width and length, and inner and outer pupal morphology changes were observed and recorded. The results show that: (i) temperature significantly influenced overall development time, as well as changes in larval width and length, but this effect was not dependent on tissue type; (ii) larval development duration was longest on heart tissue, and shortest on skeletal muscle for both temperatures; and (iii) pupation was longest for larvae reared on skeletal muscle at 24 ± 1 °C, and on liver tissue at 30 ± 1 °C, while those larvae reared on liver tissue at 24 ± 1 °C and heart tissue at 30 ± 1 °C had the shortest pupation period. A seven-character checklist plus 4 landmark stages were developed comprising the external morphology of the puparium and pupal content changes of L. sericata. In conclusion, the study provides larval and pupal development timetables, as well as checklists and photo guides for pupal character development that may be useful for future postmortem determinations.

中文翻译：

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在两种温度和三种食物类型下饲养的丝光绿蝇（双翅目：丽蝇科）幼虫和蛹的形态变化

从昆虫学角度确定最小死后间隔（minPMI）主要依赖于从犯罪现场或可疑死亡中收集的各种苍蝇的发育数据。本研究重点关注丝光绿蝇（Meigen）的幼虫和蛹发育，重点关注变态过程中蛹外部形态及其蛹内容物的变化。在 2 种不同的温度条件下，在 3 种类型的猪组织（骨骼肌、肝脏组织和心脏组织）上饲养丝光 L. sericata 菌落；24±1℃和30±1℃。观察并记录总发育时间、幼虫宽度和长度以及内外蛹形态变化。结果表明：（i）温度显着影响整个发育时间以及幼虫宽度和长度的变化，但这种影响不依赖于组织类型；(ii) 在两种温度下，幼虫发育持续时间在心脏组织上最长，在骨骼肌上最短；(iii) 在 24 ± 1 °C 的骨骼肌和 30 ± 1 °C 的肝组织上饲养的幼虫化蛹时间最长，而在 24 ± 1 °C 的肝组织和 30 ± 1 °C 的心脏组织上饲养的幼虫化蛹时间最长。 1℃时化蛹期最短。开发了一个包含 7 个字符的清单和 4 个标志性阶段，包括丝光蚕蛹的外部形态和蛹内容物的变化。总之，该研究提供了幼虫和蛹的发育时间表，以及蛹性格发育的清单和照片指南，可能对未来的尸检决定有用。