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CRISPR/Cas system: A revolutionary tool for crop improvement
Biotechnology Journal ( IF 4.7 ) Pub Date : 2024-02-26 , DOI: 10.1002/biot.202300298
Ayushi Mishra 1 , Veda P. Pandey 1
Affiliation  

World's population is elevating at an alarming rate thus, the rising demands of producing crops with better adaptability to biotic and abiotic stresses, superior nutritional as well as morphological qualities, and generation of high‐yielding varieties have led to encourage the development of new plant breeding technologies. The availability and easy accessibility of genome sequences for a number of crop plants as well as the development of various genome editing technologies such as zinc finger nucleases (ZFNs), transcription activator‐like effector nucleases (TALENs) has opened up possibilities to develop new varieties of crop plants with superior desirable traits. However, these approaches has limitation of being more expensive as well as having complex steps and time‐consuming. The CRISPR/Cas genome editing system has been intensively studied for allowing versatile target‐specific modifications of crop genome that fruitfully aid in the generation of novel varieties. It is an advanced and promising technology with the potential to meet hunger needs and contribute to food production for the ever‐growing human population. This review summarizes the usage of novel CRISPR/Cas genome editing tool for targeted crop improvement in stress resistance, yield, quality and nutritional traits in the desired crop plants.

中文翻译:

CRISPR/Cas系统:作物改良的革命性工具

世界人口正以惊人的速度增长,因此,对生产具有更好的生物和非生物胁迫适应性、优越的营养和形态品质以及高产品种的作物的需求不断增长,从而鼓励了新植物育种的发展技术。许多农作物基因组序列的可用性和易获取性,以及锌指核酸酶(ZFN)、转录激活子样效应核酸酶(TALEN)等各种基因组编辑技术的发展,为开发新品种开辟了可能性具有优良理想性状的农作物。然而,这些方法的局限性在于成本更高、步骤复杂且耗时。CRISPR/Cas 基因组编辑系统经过深入研究,可对作物基因组进行多种目标特异性修饰,从而有效地帮助新品种的产生。这是一项先进且有前途的技术,有潜力满足饥饿需求,并为不断增长的人口生产粮食做出贡献。本综述总结了新型 CRISPR/Cas 基因组编辑工具在目标作物抗逆性、产量、品质和营养性状方面的针对性改良的用途。
更新日期:2024-02-26
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