Butyl benzyl phthalate (BBP) is found in common household and industrial products world-wide. Phthalates are not covalently bound to plastics and continuously leach into the soil, sediment and aquatic environments. The lateral line system of fish is a mechanosensory system composed of neuromasts essential for survival behaviors including rheotaxis, schooling and predator avoidance. Here, we investigated the developmental toxicity of BBP on the developing lateral line neuromasts in zebrafish. Embryos were treated at gastrula stage with BBP and analyzed by DASPEI staining at 4 days post fertilization. We find that BBP negatively affects neuromast development leading to loss of DASPEI signal in neuromasts in a concentration dependent manner.

中文翻译：

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邻苯二甲酸丁苄酯 (BBP) 会破坏胚胎斑马鱼的神经丘发育

邻苯二甲酸丁苄酯 (BBP) 存在于世界各地的常见家用和工业产品中。邻苯二甲酸盐不与塑料共价结合，并不断渗入土壤、沉积物和水生环境中。鱼类的侧线系统是由神经丘组成的机械感觉系统，对于生存行为（包括趋变性、群体性和躲避捕食者）至关重要。在这里，我们研究了 BBP 对斑马鱼发育中的侧线神经丘的发育毒性。在原肠胚阶段用 BBP 处理胚胎，并在受精后 4 天通过 DASPEI 染色进行分析。我们发现 BBP 对神经丘发育产生负面影响，导致神经丘中 DASPEI 信号以浓度依赖性方式丢失。