Hypertrophic scars are usually the result of surgical trauma or burn,and more common in individuals with a darker skin color. They appear as red and raised lesions around the wound that continually expand over a period of weeks or months, causing itching, pain, burning sensation and discomfort. Severe scarring affects interpersonal and social relationships, and decreases the quality of life of the patients.The aim of this study was to evaluate the effect of carbon dioxide fractional laser as an early intervention against hypertrophic scars using a rabbit ear scar model, and explore the role of the TGFβ-1/ Smad3 signaling pathway in scar hyperplasia. Four wounds were made into each ear of rabbits, and divided into the untreated control and three laser-treatment groups. The experimental groups received laser intervention once, twice and thrice respectively. laser treatment significantly inhibited the formation of hypertrophic scars, and maximum benefits were seen in the wounds that received three laser treatments. Immunohistochemical staining showed that the in situ expression of TGFβ-1 and Smad3 in the scars decreased by varying degrees after laser intervention, and was most obvious after three laser interventions. Furthermore, the expression levels were the lowest at the end of 6 months after modeling. Therefore, we can assume that early intervention with carbon dioxide fractional laser can prevent formation of hypertrophic scars by regulating the TGF-β1/Smad3 pathway.

肥厚性疤痕通常是手术创伤或烧伤造成的，在肤色较深的人中更常见。它们表现为伤口周围红色凸起的病变，在数周或数月内不断扩大，引起瘙痒、疼痛、烧灼感和不适。严重的疤痕影响人际关系和社会关系，降低患者的生活质量。本研究的目的是利用兔耳疤痕模型评估二氧化碳点阵激光作为早期干预增生性疤痕的效果，并探讨其治疗疤痕的效果。 TGFβ-1/Smad3信号通路在疤痕增生中的作用。在兔子的每只耳朵上制作四个伤口，并将其分为未治疗的对照组和三个激光治疗组。实验组分别接受1次、2次、3次激光干预。激光治疗显着抑制了增生性疤痕的形成，并且在接受三种激光治疗的伤口中看到了最大的益处。免疫组化染色显示，激光干预后疤痕内TGFβ-1和Smad3的原位表达均有不同程度下降，且在3次激光干预后最为明显。此外，在造模后6个月末表达水平最低。因此，我们可以假设二氧化碳点阵激光的早期干预可以通过调节TGF-β1/Smad3通路来预防增生性疤痕的形成。