Anti-Ferroptotic Effect of Cannabidiol in Human Skin Keratinocytes Characterized by Data-Independent Acquisition-Based Proteomics,Journal of Natural Products

当前位置： X-MOL 学术 › J. Nat. Prod. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Anti-Ferroptotic Effect of Cannabidiol in Human Skin Keratinocytes Characterized by Data-Independent Acquisition-Based Proteomics
Journal of Natural Products ( IF 3.3 ) Pub Date : 2024-02-19 , DOI: 10.1021/acs.jnatprod.3c00759
Huifang Li ₁ , Tess Puopolo ₁ , Navindra P Seeram ₁ , Chang Liu ₁ , Hang Ma ₁

Affiliation

Skin cells are susceptible to oxidative stress and various types of cell death, including an iron-dependent form known as ferroptosis. Cannabidiol (CBD) can protect skin cells against oxidative stress, but whether this is attributed to the inhibition of ferroptosis is unknown. Herein, we evaluated the anti-ferroptotic effect of CBD in human keratinocytes using biochemical assays (radical scavenging and iron chelating) and cell-based models (for lipid peroxidation and intracellular iron). CBD’s anti-ferroptotic effect was further characterized by proteomic analysis. This study identifies anti-ferroptosis as a mechanism of CBD’s skin protective effects.

中文翻译：

大麻二酚对人皮肤角质形成细胞的抗铁死亡作用，以数据独立的基于采集的蛋白质组学为特征

皮肤细胞容易受到氧化应激和各种类型的细胞死亡的影响，包括一种称为铁死亡的铁依赖性形式。大麻二酚 (CBD) 可以保护皮肤细胞免受氧化应激，但这是否归因于铁死亡的抑制尚不清楚。在此，我们使用生化测定（自由基清除和铁螯合）和基于细胞的模型（脂质过氧化和细胞内铁）评估了 CBD 在人类角质形成细胞中的抗铁焦亡作用。通过蛋白质组学分析进一步表征了 CBD 的抗铁死亡作用。这项研究确定抗铁死亡是 CBD 皮肤保护作用的机制。

更新日期：2024-02-19

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11