The aim of the study was to investigate the impact of multiwave locked system (MLS M1) emitting synchronized laser radiation at 2 wavelength simultaneous (λ = 808 nm, λ = 905 nm) on the mesenchymal stem cells (MSCs). Human MSCs were exposed to MLS M1 system laser radiation with the power density 195–318 mW/cm² and doses of energy 3–20 J, in continuous wave emission (CW) or pulsed emission (PE). After irradiation exposure in doses of energy 3 J, 10 J (CW, ƒ = 1000 Hz), and 20 J (ƒ = 2000 Hz), increased proliferation of MSCs was observed. Significant reduction of Fluo-4 Direct™ Ca²⁺ indicator fluorescence over controls after CW and PE with 3 J, 10 J, and 20 J was noticed. A decrease in fluorescence intensity after the application of radiation with a frequency of 2000 Hz in doses of 3 J, 10 J, and 20 J was observed. In contrary, an increase in DCF fluorescence intensity after irradiation with laser radiation of 3 J, 10 J, and 20 J (CW, ƒ = 1000 Hz and ƒ = 2000 Hz) was also shown. Laser irradiation at a dose of 20 J, emitted at 1000 Hz and 2000 Hz, and 3 J emitted at a frequency of 2000 Hz caused a statistically significant loss of MSC viability. The applied photobiomodulation therapy induced a strong pro-apoptotic effect dependent on the laser irradiation exposure time, while the application of a sufficiently high-energy dose and frequency with a sufficiently long exposure time significantly increased intracellular calcium ion concentration and free radical production by MSCs.

本研究的目的是研究多波锁定系统 (MLS M1) 同时发射 2 个波长（ λ  = 808 nm、λ  = 905 nm）的同步激光辐射对间充质干细胞 (MSC)的影响。将人类 MSC 暴露于 MLS M1 系统激光辐射下，功率密度为 195-318 mW/cm ²，能量剂量为 3-20 J，采用连续波发射 (CW) 或脉冲发射 (PE)。在能量剂量为 3 J、10 J（CW，f = 1000 Hz）和 20 J（f = 2000 Hz）的照射后，观察到 MSC 增殖增加。注意到在 CW 和 PE（3 J、10 J 和 20 J）后， Fluo-4 Direct™ Ca ²⁺指示剂荧光显着降低。在施加频率为 2000 Hz、剂量为 3 J、10 J 和 20 J 的辐射后，观察到荧光强度降低。相反，还显示出用3J、10J和20J(CW，f＝1000Hz和f＝2000Hz)的激光辐射照射后DCF荧光强度增加。剂量为 20 J、频率为 1000 Hz 和 2000 Hz 的激光照射以及频率为 2000 Hz 的 3 J 的激光照射导致 MSC 活力出现统计上显着的损失。所应用的光生物调节疗法诱导了依赖于激光照射暴露时间的强烈促凋亡作用，而应用足够高的能量剂量和频率以及足够长的暴露时间显着增加了MSCs的细胞内钙离子浓度和自由基产生。