Although abnormally fertilized zygotes with three or multiple pronuclei (3 PN/MPN) are commonly believed to be associated with improper maturation of the oocyte cytoplasm in conventional IVF cycles, no studies investigated the association between the proportion of MPN zygotes and the maturation state of the oocyte cohort. We compared the cytoplasmic maturity of oocytes from conventional IVF cycles with different proportions of 3 PN/MPN zygotes. A total of 1428 conventional IVF patients with ≥6 oocytes retrieved and fresh embryos transferred were divided into 4 groups according to the proportions of 3 PN/MPN zygotes. The pregnancy outcomes and the proportion of nuclear immature oocytes were analyzed to suggest the cytoplasmic maturation state of the oocyte cohort. Our results showed that the group with a low proportion of 3 PN/MPN zygotes had a higher clinical pregnancy rate (CPR) than those without 3 PN/MPN zygotes ( < 0.05). However, the live birth rate (LBR) was not significantly different between the two groups. The implantation rate (IR), CPR, and LBR did not differ between the low-proportion and high-proportion 3 PN/MPN groups. The proportion of nuclear immature oocytes on day 1 was highest in the group without 3 PN/MPN zygotes (23.8 %) and gradually decreased with an increased proportion of 3 PN/MPN zygotes ( < 0.001). Therefore, the presence of 3 PN/MPN zygotes after conventional IVF may indicate a more mature cytoplasmic state of the oocyte cohort, and the increased proportion of 3 PN/MPN zygotes is associated with an increased maturation state of the whole oocyte cohort. The occurrence and proportion of 3 PN/MPN zygotes may serve as an indicator for the cytoplasmic maturity of the oocyte cohort and help clinicians evaluate the efficiency of ovarian stimulation and optimize the stimulation protocols in subsequent cycles.

中文翻译：

---

三/多原核（3PN/MPN）受精卵的存在和比例能否表明传统 IVF 患者卵母细胞群的细胞质成熟状态？

尽管通常认为具有三个或多个原核（3 PN/MPN）的异常受精受精卵与传统 IVF 周期中卵母细胞细胞质成熟不当有关，但没有研究调查 MPN 受精卵的比例与卵母细胞成熟状态之间的关系。卵母细胞队列。我们比较了传统 IVF 周期中不同比例的 3 PN/MPN 受精卵的卵母细胞的细胞质成熟度。选取≥6个卵母细胞并移植新鲜胚胎的常规IVF患者1428例，根据3个PN/MPN受精卵的比例分为4组。分析妊娠结局和核未成熟卵母细胞的比例，以提示卵母细胞群的细胞质成熟状态。我们的结果显示，3个PN/MPN受精卵比例较低的群体比没有3个PN/MPN受精卵的群体具有更高的临床妊娠率（CPR）（<0.05）。然而，两组之间的活产率（LBR）没有显着差异。低比例和高比例 3 PN/MPN 组的着床率 (IR)、CPR 和 LBR 没有差异。第1天核未成熟卵母细胞的比例在没有3个PN/MPN受精卵的组中最高(23.8%)，并且随着3个PN/MPN受精卵比例的增加而逐渐下降(<0.001)。因此，常规IVF后3个PN/MPN合子的存在可能表明卵母细胞群的细胞质状态更加成熟，并且3个PN/MPN合子比例的增加与整个卵母细胞群的成熟状态的增加相关。 3个PN/MPN合子的出现和比例可以作为卵母细胞群细胞质成熟度的指标，帮助临床医生评估卵巢刺激的效率并优化后续周期的刺激方案。