Chemical investigation of Irpex sp. NBUF088, associated with an Ircinia sp. sponge located at an 84 m deep mesophotic zone, led to the discovery of two new heptaketides, named irpetones A (1) and B (2). Their structures were identified by analysis of spectroscopic data and quantum-chemical calculations. Compound 1 exhibited inhibition against the receptor activator of NF-κB ligand-induced osteoclastogenesis in bone marrow monocytes with an IC₅₀ of 6.3 ± 0.2 μM, causing no notable cytotoxicity. It was also determined that 1 inhibited the phosphorylation of ERK1/2-JNK1/2-p38 MAPKs and the nuclear translocation of NF-κB, consequently suppressing the activation of MAPK and NF-κB signaling pathways induced by the NF-κB ligand.

中文翻译：

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Irpetones A 和 B，来自海洋中光带 Ircinia 海绵相关真菌 Irpex sp. 的抗破骨七肽。 NBUF088

Irpex sp的化学研究。 NBUF088，与Ircinia sp.相关。位于84 m深的中光带的海绵，导致发现了两个新的七肽，命名为irpetones A ( 1 )和B ( 2 )。通过分析光谱数据和量子化学计算来鉴定它们的结构。化合物1对骨髓单核细胞中 NF-κB 配体诱导的破骨细胞生成的受体激活剂具有抑制作用，IC ₅₀为 6.3 ± 0.2 μM，没有引起明显的细胞毒性。还确定1抑制 ERK1/2-JNK1/2-p38 MAPK 的磷酸化和 NF-κB 的核转位，从而抑制 NF-κB 配体诱导的 MAPK 和 NF-κB 信号通路的激活。