New psychoactive substances are produced and marketed to mimic the effects of their illicit counterparts and to attempt to evade drug tests and prosecution. Here, we present the optimisation, validation and application of an analytical method using liquid chromatography–time-of-flight mass spectrometry to detect and quantify 37 new psychoactive substances and illicit substances in wastewater from South Wales, UK, using a targeted analysis method. Sample preparation was performed using solid-phase extraction with Oasis HLB cartridges. The LC separation was performed using a YMC-Triart Phenyl 450 bar column (12 nm, 5 μm, 100 × 3 mm) which provided good separation and resolution for all targeted analytes with a run time of 9 min. The method was validated using the following parameters: sensitivity, selectivity, linearity, accuracy, precision, recovery and matrix effects. The method was then applied to influent wastewater samples collected from two wastewater treatment plants in Wales, UK.

中文翻译：

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通过优化的液相色谱-飞行时间分析显示英国南威尔士废水中不存在新的精神活性物质

新的精神活性物质的生产和销售是为了模仿其非法同类物质的效果并试图逃避药物测试和起诉。在这里，我们介绍了一种使用液相色谱-飞行时间质谱分析方法的优化、验证和应用，以有针对性的分析方法检测和定量英国南威尔士废水中的 37 种新的精神活性物质和非法物质。使用 Oasis HLB 柱进行固相萃取进行样品制备。使用 YMC-Triart Phenyl 450 bar 色谱柱（12 nm、5 μm、100 × 3 mm）进行 LC 分离，该色谱柱为所有目标分析物提供了良好的分离和分辨率，运行时间为 9 分钟。该方法使用以下参数进行验证：灵敏度、选择性、线性、准确度、精密度、回收率和基质效应。然后将该方法应用于从英国威尔士的两个废水处理厂收集的进水废水样品。