Disulfide bridges (DSB) play an important role in stabilizing three-dimensional structures of biopharmaceuticals, single purified proteins, and various cyclic peptide drugs that contain disulfide in their structures. Incorrect cross-linking known as DSB scrambling results in misfolded structures that can be inactive, immunogenic, and susceptible to aggregation. Very few articles have been published on the experimental annotation of DSBs in proteins and cyclic peptide drugs. Accurate characterization of the disulfide bond is essential for understanding protein confirmation.

中文翻译：

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二硫桥表征的最新进展：来自质谱的见解

二硫桥（DSB）在稳定生物制药、单一纯化蛋白质以及结构中含有二硫键的各种环肽药物的三维结构方面发挥着重要作用。不正确的交联（称为 DSB 扰乱）会导致错误折叠的结构，这些结构可能无活性、具有免疫原性且易于聚集。关于蛋白质和环肽药物中 DSB 的实验注释发表的文章很少。二硫键的准确表征对于理解蛋白质的确认至关重要。