Abstract

The purpose of this research was to characterize the microbiota of patients with psoriatic arthritis (PsA) and to compare the relationship between the microbiota and peripheral lymphocyte subsets and cytokines. We collected stool samples from 13 PsA patients and 26 sex- and age-matched healthy controls (HCs) and researched the gut microbiota by sequencing the V3-V4 variable region of the bacterial 16S rRNA gene with the Illumina Miseq PE300 system. Flow cytometry was used to assess the peripheral lymphocyte subsets in these participants. Record measures of disease activity such as C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR). Alpha and beta diversity were assessed using results from QIIME2. Panel demonstrated the average relative abundance of the different genera in PsA and HCs. Correlation between clinical parameters and the relative abundance of the genus in samples was assessed by the Pearson correlation analysis using R (version 4.0.1). Compared with HC, the abundance of gut microbiota (Chao 1 and ACE) decreased in patients with PsA, and the diversity of bacteria (Shannon and Simpson indices) also decreased in PsA (Fig. 1a). β Diversity analysis indicated differences in microbial communities between PsA and HC (Fig. 1b, r = 0.039, p = 0.264, ANOSIM). Furthermore, 18 bacterial groups were significantly different at the genus level in PsA compared to HCs (p < 0.05) (Fig. 2).In the phylum and genus, lymphocyte subsets and cytokines are associated with the microbiota. The gut microbiota of patients with PsA differs from HC, which was closely related to lymphocyte subsets.

中文翻译：

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银屑病关节炎患者肠道菌群失调与淋巴细胞亚群和细胞因子密切相关

摘要

本研究的目的是描述银屑病关节炎 (PsA) 患者的微生物群特征，并比较微生物群与外周淋巴细胞亚群和细胞因子之间的关系。我们收集了 13 名 PsA 患者和 26 名性别和年龄匹配的健康对照 (HC) 的粪便样本，并通过使用 Illumina Miseq PE300 系统对细菌 16S rRNA 基因的 V3-V4 可变区进行测序来研究肠道微生物群。使用流式细胞术评估这些参与者的外周淋巴细胞亚群。记录疾病活动的测量结果，例如 C 反应蛋白 (CRP) 和红细胞沉降率 (ESR)。使用 QIIME2 的结果评估 Alpha 和 Beta 多样性。面板显示了 PsA 和 HC 中不同属的平均相对丰度。使用 R（版本 4.0.1）通过 Pearson 相关分析评估临床参数与样本中该属相对丰度之间的相关性。与HC相比，PsA患者肠道微生物群（Chao 1和ACE）丰度下降，细菌多样性（Shannon指数和Simpson指数）也下降（图 1a）。 β多样性分析表明PsA和HC之间微生物群落存在差异（图 1b，r = 0.039，p  = 0.264，ANOSIM）。此外，与 HC 相比，PsA 中的 18 个细菌群在属水平上存在显着差异（p  < 0.05）（图 2）。在门和属中，淋巴细胞亚群和细胞因子与微生物群相关。 PsA患者的肠道菌群与HC患者不同，与淋巴细胞亚群密切相关。