Background

Salt (NaCl) promotes T-lymphocyte conversion to pro-inflammatory Th-17 cells in vitro. Interleukin (IL)-17A aggravates hypertension in preeclampsia (PE) models.

Objectives

It was hypothesized that 1) women with PE exhibit increased plasma IL-17A and related cytokines and 2) high dietary salt intake elevates circulating IL-17A in patients with PE compared to women with healthy pregnancy (HP) and non-pregnant (NonP) women.

Main outcome measures

Plasma concentration of cytokines IL-17A, IFN-γ, IL-10, TNF, IL-6, and IL-1β in samples from NonP women (n = 13), HP (n = 15), and women with PE (n = 7).

Study Design

Biobanked samples from a randomized, double-blind, cross-over placebo-controlled dietary intervention study. Participants received a low sodium diet (50–60 mmol NaCl/24 h) for 10 days and were randomly assigned to ingest placebo tablets (low salt intake) or salt tablets (172 mmol NaCl/24 h, high salt intake) for 5 + 5 days. Plasma samples were drawn at baseline and after each diet.

Results

While a high salt diet suppressed renin, angiotensin II, and aldosterone levels, it did not affect blood pressure or plasma cytokine concentrations in any group compared to low salt intake. Plasma TNF was significantly higher in PE than in HP and NonP at baseline and after a low salt diet. Plasma IL-6 was significantly higher in PE compared to HP at baseline and NonP at low salt.

Conclusion

Interleukin-17A and related T-cell and macrophage-cytokines are not sensitive to salt-intake in PE. Preeclampsia is associated with elevated levels of TNF and IL-6 macrophage-derived cytokines. Salt-sensitive changes in systemic IL-17A are less likely to explain hypertension in PE.

中文翻译：

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盐摄入量的短期变化对健康妊娠和高血压妊娠女性血浆细胞因子的影响

背景

盐 (NaCl)在体外促进 T 淋巴细胞转化为促炎 Th-17 细胞。白细胞介素 (IL)-17A 会加重先兆子痫 (PE) 模型中的高血压。

目标

假设 1) 患有 PE 的女性血浆 IL-17A 和相关细胞因子增加，2) 与健康妊娠 (HP) 和非妊娠 (NonP) 的女性相比，高饮食盐摄入会升高 PE 患者的循环 IL-17A女性。

主要观察指标

非 P 女性 ( n  = 13)、HP ( n  = 15) 和 PE 女性 ( n ) 样本中细胞因子 IL-17A、IFN-γ、IL-10、TNF、IL-6 和 IL-1β 的血浆浓度 = 7）。

学习规划

生物库样本来自一项随机、双盲、交叉安慰剂对照饮食干预研究。参与者接受低钠饮食（50-60 mmol NaCl/24 小时）10 天，并被随机分配服用安慰剂片（低盐摄入量）或盐片（172 mmol NaCl/24 小时，高盐摄入量）5+ 5天。在基线和每次饮食后抽取血浆样本。

结果

虽然高盐饮食抑制肾素、血管紧张素 II 和醛固酮水平，但与低盐摄入相比，它不会影响任何组的血压或血浆细胞因子浓度。在基线和低盐饮食后，PE 中的血浆 TNF 显着高于 HP 和 NonP。与基线时的 HP 和低盐时的 NonP 相比，PE 中的血浆 IL-6 显着较高。

结论

白细胞介素 17A 和相关 T 细胞和巨噬细胞细胞因子对 PE 中的盐摄入不敏感。先兆子痫与 TNF 和 IL-6 巨噬细胞衍生的细胞因子水平升高有关。全身 IL-17A 的盐敏感性变化不太可能解释 PE 中的高血压。