Introduction

: Persicaria amphibia L. (water knotweed), an edible plant with pharmaceutical properties, is found in Asia, Europe, North America, and Africa. Despite its worldwide distribution and prominence, scientific literature on the plant's therapeutic properties is scarce. This study aimed to comprehensively investigate the antioxidant activity, DNA binding potential, GC-MS, and fluorescence analysis and photoprotective properties of P. amphibia.

Method

: Water and ethanol maceration and Soxhlet extraction samples (Pa1-4) of P. amphibia were prepared. Bioactive properties of the plant were investigated. Qualitative and quantitative chemical markers of the samples were described using UV-Vis., fluorescence, and gas chromatography-mass spectrometry (GC-MS) analyses, and phytochemical screening. Photoprotective properties of the plant were also revealed using Mansur method.

Results

: The phenolic and flavonoid contents varied between 22.06 ± 0.49 and 28.92 ± 0.51 μg gallic acid equivalent (GAE)/1 mg and 24.54 ± 1.19 and 34.09 ± 0.61 μg quercetin equivalent (QE)/1 mg, respectively. The fluorescence emission spectra of the samples indicated that the samples may contain berberine alkaloid, and riboflavin and quercetin flavonoids. The sun protection factor (SPF) values revealed that the plant [Pa1 (750 and 1000 μg/mL = 10.22 ± 0.05; 13.29 ± 0.25), Pa2 (500 μg/mL = 7.58 ± 0.04) and Pa4 (1000 μg/mL: 11.86 ± 0.13)] has more photoprotective activity compared with carrot seed oil (500, 750 and 1000 µg/mL = 5.22 ± 0.07; 7.72 ± 0.09 and 9.69 ± 0.14) (P ˂ 0.001). Moreover, the samples exhibited considerable antioxidant activity. All four extracts could generally inhibit lipid peroxidation (Pa1-4 = 83.14% ± 0.36; 82.05 % ± 0.82; 80.87% ± 0.23 and 81.38% ± 1.26) as effectively as the standards of ascorbic acid (81.89% ± 0.88) and α-tocopherol (85.45 ± 1.37) for 25 µg/mL (P ˃ 0.05). The binding affinities between the plant samples and Calf Thymus DNA (CT-DNA) were also determined. The order of the binding affinity is Pa3 > Pa4 > Pa2 > Pa1.

Conclusion

: The P. amphibia plant has a significant amount and variety of phytocomponents. Along with bioactive and sun protection properties, this plant could be a cheap, beneficial, and safe ingredient for cosmetic and pharmaceutical formulations.

中文翻译：

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水虎杖因其高光防护性和显着的生物活性而成为临床前试验的重要候选物

介绍

: 两栖蟋蟀L . （水虎杖）是一种具有药用价值的食用植物，分布于亚洲、欧洲、北美和非洲。尽管其在世界范围内分布广泛且知名度很高，但有关该植物治疗特性的科学文献却很少。本研究旨在综合研究两栖类P. amphibia的抗氧化活性、DNA 结合电位、GC-MS、荧光分析和光保护特性。

方法

：制备两栖类双歧杆菌的水和乙醇浸渍和索氏提取样品（Pa1-4） 。研究了该植物的生物活性特性。使用紫外-可见光、荧光、气相色谱-质谱（GC-MS）分析和植物化学筛选来描述样品的定性和定量化学标记物。使用曼苏尔方法还揭示了该植物的光保护特性。

结果

：酚类和类黄酮含量变化范围分别为 22.06 ± 0.49 和 28.92 ± 0.51 μg 没食子酸当量 (GAE)/1 mg 以及 24.54 ± 1.19 和 34.09 ± 0.61 μg 槲皮素当量 (QE)/1 mg。样品的荧光发射光谱表明样品中可能含有小檗碱生物碱、核黄素和槲皮素类黄酮。防晒系数 (SPF) 值显示植物 [ Pa1（750 和 1000 μg/mL = 10.22 ± 0.05；13.29 ± 0.25）、Pa2（500 μg/mL = 7.58 ± 0.04）和Pa4（1000 μg/mL： 11.86 ± 0.13)] 与胡萝卜籽油相比具有更高的光保护活性（500、750 和 1000 µg/mL = 5.22 ± 0.07；7.72 ± 0.09 和 9.69 ± 0.14）（P ˂ 0.001）。此外，样品表现出相当大的抗氧化活性。所有四种提取物通常都能抑制脂质过氧化（Pa1-4  = 83.14%±0.36；82.05%±0.82；80.87%±0.23和81.38%±1.26），与抗坏血酸（81.89%±0.88）和α-标准品一样有效。生育酚 (85.45 ± 1.37) 为 25 µg/mL ( P ˃ 0.05)。还测定了植物样品和小牛胸腺 DNA (CT-DNA) 之间的结合亲和力。结合亲和力的顺序是Pa3 > Pa4 > Pa2 > Pa1。

结论

：两栖类植物含有大量且种类丰富的植物成分。除了生物活性和防晒特性之外，这种植物可以成为化妆品和药物配方中廉价、有益且安全的成分。