This study describes a novel application for sandpaper spray ionization mass spectrometry (SPS-MS), to examine the surface of maple tree (Acer sp.) leaves. By comparing mass spectrometry fingerprints, healthy leaves from those infected with powdery mildew and Rhytisma acerinum were distinguished. Leaves were grated with sandpaper, cut into triangles, and placed before the mass spectrometer, with the addition of a methanol-formic acid solution. Multivariate statistical analysis categorized the samples into three groups. Overall, SPS-MS effectively analyzed leaves with infectious microorganisms, potentially aiding in the creation of fungal identification databanks.

中文翻译：

---

应用砂纸喷雾电离质谱法综合检测不同真菌感染枫叶

本研究描述了砂纸喷雾电离质谱 (SPS-MS) 的一种新应用，用于检查枫树 ( Acer sp.) 叶子的表面。通过比较质谱指纹图谱，区分健康叶子和感染白粉病和Rhytisma acerinum的叶子。将叶子用砂纸磨碎，切成三角形，并放置在质谱仪前，并添加甲醇-甲酸溶液。多元统计分析将样本分为三组。总体而言，SPS-MS 有效地分析了含有传染性微生物的叶子，可能有助于创建真菌鉴定数据库。