The laboratory practice “Primary culture and directional differentiation of rat bone marrow mesenchymal stem cells (BMSCs)” is part of a required course for sophomore medical students at Tongji university, which has been conducted since 2012. Blended learning has been widely applied in medical courses. Based on a student-centered teaching philosophy, we reconstructed a comprehensive stem cell laboratory module with blended learning in 2021, aiming to facilitate students in enhancing their understanding of the multi-lineage differentiation potential of stem cells and improve their experimental skills, self-directed learning ability, and innovative thinking. First, we constructed in-depth online study resources, including videos demonstrating laboratory procedures, a PowerPoint slide deck, and published literature on student self-learning before class. In class, students performed a primary culture of BMSCs, freely chose among adipogenic, osteogenic, or chondrogenic differentiation, and used cytochemical or immunofluorescence staining for identification. After class, the extracurricular part involved performing quantitative polymerase chain reaction to examine the expression of multi-lineage differentiation marker genes, which was designed as an elective. After 2 years of practice, positive feedback was obtained from both students and faculty members who achieved, the learning goal as expected. The reconstructed stem cell laboratory module provides comprehensive practice opportunities for students. Students have a better understanding of BMSC at the molecular, cellular, and functional levels and have improved their experimental skills, which forms a basis for scientific research for medical students. Introducing blended learning into other medical laboratory practices thus seems valuable.

中文翻译：

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同济大学医学生混合式学习的综合干细胞实验室模块

《大鼠骨髓间充质干细胞（BMSCs）的原代培养与定向分化》实验室实践是同济大学医学大二学生必修课，自2012年开始开设，混合式学习在医学课程中得到广泛应用。本着以学生为中心的教学理念，我们于2021年重构了混合式学习的综合性干细胞实验室模块，旨在帮助学生增强对干细胞多谱系分化潜能的认识，提高实验技能、自主学习能力。学习能力、创新思维。首先，我们构建了深入的在线学习资源，包括演示实验室程序的视频、PowerPoint 幻灯片以及关于学生课前自学的文献。课堂上，学生对BMSC进行原代培养，自由选择成脂、成骨或软骨分化，并使用细胞化学或免疫荧光染色进行鉴定。课后，课外部分是进行定量聚合酶链反应检测多谱系分化标记基因的表达，被设计为选修课。经过两年的实践，得到了学生和教师的积极反馈，达到了预期的学习目标。重建的干细胞实验室模块为学生提供了综合实践机会。学生对骨髓间充质干细胞在分子、细胞和功能层面有了更深入的了解，提高了实验技能，为医学生的科学研究奠定了基础。因此，将混合式学习引入其他医学实验室实践似乎很有价值。