Wilt disease complex of carrot is caused by Ralstonia solancearum and Meloidogyne incognita and is responsible for considerable yield loss. Manganese oxide nanoparticle (MnO₂ NPs) and Pseudomonas putida were used alone and in combination for the management of wilt disease complex. In vitro, MnO₂ NPs 0.10 g.L^-l caused 49.36% reduction in hatching and 14.23% mortality of second stage juveniles (J₂) of M. incognita while paper disc dipped in MnO₂ NPs suspension caused 0.51 mm inhibition zone around R. solanacearum in nutrient agar medium. Inoculation of P. putida to plants with pathogens caused a similar increase in plant growth, chlorophyll and carotenoid contents as caused by foliar spray with 0.10 g.L⁻¹ MnO₂ NPs. Use of P. putida with NPs foliar spray to plants with pathogens caused a greater increase in plant growth, chlorophyll and carotenoid contents than with P. putida or NPs foliar spray. Inoculation of M. incognita/R. solanacearum/M. incognita plus R. solanacearum/P. putida/MnO₂ NPs and MnO₂ NPs plus P. putida caused increase in proline contents. Root colonization by P. putida was reduced in plants with test pathogens. Foliar application of MnO₂ NPs and P. putida reduce wilt disease indices. Galling and populations of M. incognita was also reduced in plants co-inoculated with R. solanacearum. The greatest reduction in nematode populations and galling was observed in plants with NPs spray together with P. putida. Principal component analysis demonstrated a clear influence of NPS and P. putida and their combination on various studied parameters in diseased plants.

胡萝卜枯萎病复合体由青枯雷尔斯顿菌和南方根结线虫引起，造成相当大的产量损失。氧化锰纳米颗粒(MnO ₂ NPs)和恶臭假单胞菌单独或联合使用用于防治枯萎病复合体。体外实验中，MnO ₂ NPs 0.10 gL ^-l导致南方根结线虫第二期幼虫（J ₂）孵化率下降49.36%，死亡率降低14.23% ，而纸片浸入MnO _{2 NPs悬浮液后，在}青枯菌周围形成0.51 mm的抑菌圈。在营养琼脂培养基中。将恶臭假单胞菌接种到带有病原体的植物上引起与叶面喷洒0.10gL ^-1 MnO ₂ NPs引起的植物生长、叶绿素和类胡萝卜素含量类似的增加。与使用恶臭假单胞菌或 NPs 叶面喷雾相比，使用恶臭假单胞菌和 NPs 叶面喷雾对带有病原体的植物造成植物生长、叶绿素和类胡萝卜素含量的更大增加。接种M. incognita / R. solanacearum / M. incognita加R. solanacearum/P. incognita 恶臭假单胞菌/ MnO ₂ NPs和MnO ₂ NPs加上恶臭假单胞菌导致脯氨酸含量增加。在带有测试病原体的植物中，恶臭假单胞菌的根部定殖减少。叶面喷施MnO ₂ NPs和恶臭假单胞菌可降低枯萎病指数。在与青枯菌共同接种的植物中，根结线虫的瘿病和种群数量也有所减少。在同时喷洒纳米颗粒和恶臭假单胞菌的植物中，观察到线虫数量和虫瘿现象的最大减少。主成分分析表明，NPS 和恶臭假单胞菌及其组合对患病植物的各种研究参数具有明显的影响。