Carbofuran is one of the most toxic broad-spectrum pesticides. We evaluated the effects of carbofuran on two species of microalgae, Pseudopediastrum boryanum and Desmodesmus communis, through measurements of cell viability, biomass, chlorophyll content, and the production of reactive oxygen species (ROS). The ability of these algae to remove carbofuran dissolved in the media was also determined. For the evaluations, both microalgae species were exposed to carbofuran (FURADAN 350 SC®) at concentrations of 100, 1000, and 10,000 µg L^–1 for 7 days. Algae cell viability and chlorophyll-a concentration were not affected by the presence of carbofuran. Both species grew when exposed to the pesticide; however, the microalgae D. communis grew less than its respective control when exposed to the highest concentration (10,000 µg L^–1 of carbofuran), indicating an adverse effect of the pesticide on this species. A significant increase in ROS production was observed in D. communis and P. boryanum when exposed to the highest concentration tested. The microalgae P. boryanum completely removed carbofuran in the media within 2 days, regardless of the concentration, whereas D. communis achieved the same result only after 5 days of exposure. Growth inhibition was observed only until the disappearance of carbofuran from the media. The present study suggests the use of microalgae, mainly P. boryanum, as potential tools for the remediation of environments contaminated by carbofuran because of their resistance to the insecticide and their ability to remove it rapidly from water. Environ Toxicol Chem 2024;43:926–937. © 2023 SETAC

中文翻译：

---

农药克百威对两种绿藻（链丝藻和硼假黄藻）的影响及其农药生物修复能力

呋喃丹是毒性最强的广谱农药之一。我们通过测量细胞活力、生物量、叶绿素含量和活性氧 (ROS) 的产生，评估了克百威对两种微藻（Pseudodiastrum boryanum和Desmodesmus commis ）的影响。还测定了这些藻类去除溶解在介质中的克百威的能力。为了进行评估，两种微藻物种均暴露于浓度为 100、1000 和 10,000 µg L ^–1的呋喃丹 (FURADAN 350 SC®)中 7 天。藻类细胞活力和叶绿素-a浓度不受呋喃丹存在的影响。两种物种在接触农药后都会生长。然而，当暴露于最高浓度（10,000 µg L ^–1克百威）时，微藻D. commis 的生长速度低于其各自的对照，表明该农药对该物种产生了不利影响。当暴露于最高测试浓度时，在D. communis和P. boryanum中观察到 ROS 产量显着增加。无论浓度如何，微藻P. boryanum在 2 天内完全去除了培养基中的克百威，而D. commis仅在暴露 5 天后才达到相同的结果。仅在呋喃丹从培养基中消失之前才观察到生长抑制。本研究建议使用微藻（主要是硼酸藻）作为修复克百威污染环境的潜在工具，因为它们对杀虫剂具有抗性，并且能够快速从水中去除杀虫剂。环境毒理学化学2024；43：926–937。 © 2023 SETAC 版权所有