Cell behaviors are highly sensitive to the surrounding environment. Therefore, in regulating cells, biomaterial substrates should be designed so their properties are similar to the surrounding environments of the cells. For cell regulation, we designed dual stimuli-responsive gels whose physical (elastic modulus) and chemical (hydrophilicity) properties can be changed by varying the UV exposure time and temperature, respectively. A dual stimuli-responsive polymer with photodimerizable groups and temperature-responsive moieties was prepared by copolymerizing 7-methacryloyloxycoumarin (MAC) and methoxyoligoethylene glycol methacrylate (OEGMA). The resulting polymers (P(MAC-co-OEGMA)) had lower critical solution temperatures (LCSTs), which depended on the compositions. A buffer solution containing P(MAC-co-OEGMA) was exposed to ultraviolet light (UV) for gelation, and the elastic modulus increased with increasing exposure time. The cell behavior, including adhesion and spreading, on the surfaces of these gels was investigated. Most of the cells adhered to P(MAC-co-OEGMA) gels with higher elastic moduli, and the cells were spread more effectively at temperatures above the LCST. This was because cell adhesion and spreading were strongly influenced by the physical and chemical properties of the P(MAC-co-OEGMA) gels, which were regulated by varying the UV exposure time and temperature.

细胞行为对周围环境高度敏感。因此，在调节细胞时，生物材料基底的设计应使其特性与细胞周围环境相似。对于细胞调节，我们设计了双重刺激响应凝胶，其物理（弹性模量）和化学（亲水性）特性可以分别通过改变紫外线暴露时间和温度来改变。通过共聚 7-甲基丙烯酰氧基香豆素 (MAC) 和甲氧基低聚乙二醇甲基丙烯酸酯 (OEGMA)，制备了具有光二聚基团和温度响应部分的双刺激响应聚合物。所得聚合物 (P(MAC- co -OEGMA)) 具有较低的临界溶解温度 (LCST)，这取决于组成。将含有P(MAC- co -OEGMA)的缓冲溶液暴露于紫外光(UV)下进行凝胶化，并且弹性模量随着暴露时间的增加而增加。研究了这些凝胶表面上的细胞行为，包括粘附和铺展。大多数细胞粘附在具有较高弹性模量的P(MAC- co -OEGMA)凝胶上，并且在高于LCST的温度下细胞更有效地铺展。这是因为细胞粘附和铺展受到 P(MAC- co -OEGMA) 凝胶的物理和化学性质的强烈影响，而这些性质通过改变紫外线暴露时间和温度来调节。