Recently, metabolome analysis has been applied to a variety of research fields, but differences between batches or facilities can cause discrepancies in the results of such analyses. To resolve these issues using comprehensive metabolome analysis, in which it is difficult to perform quantitative analyses of all detected metabolites, internal standard compounds are used to obtain relative metabolite levels. This study investigated gas chromatography/mass spectrometry–based plasma metabolome analysis methods that are superior to relative quantification using internal standard compounds.

中文翻译：

---

用户友好的相对定量程序，用于基于气相色谱/质谱的血浆代谢组分析

近年来，代谢组分析已应用于多种研究领域，但批次或设施之间的差异可能会导致此类分析结果的差异。为了使用综合代谢组分析解决这些问题，其中很难对所有检测到的代谢物进行定量分析，使用内标化合物来获得相对代谢物水平。本研究研究了基于气相色谱/质谱的血浆代谢组分析方法，该方法优于使用内标化合物进行相对定量。