Shedder status is defined as the propensity of an individual to leave DNA behind on touched items or surfaces and has been suggested as one of the major factors influencing DNA transfer. However, little is known about whether shedder status is a constant property of an individual across multiple measurements or when the environmental conditions are changed. We have assessed DNA depositions of six males on 20 occasions to acquire a reference data set and to classify the participants into high, intermediate, or low shedders. This data set was also used to investigate how the probability of a correct shedder status classification changed when the number of DNA deposition measurements increased. Individual sweat rates were measured with a VapoMeter and data regarding hygiene routines were collected through a questionnaire on each sampling occasion. Next, we investigated how changes in the experimental conditions such as seasonal variation, hygiene routines, the temperature of the touched object, and repeated handling of an object influenced the DNA shedding. Additionally, we assessed DNA collected from the face and from T-shirts worn by the six participants to explore whether shedder status may be associated with the relative amount of DNA obtained from other body parts. Our results indicate that shedder status is a stable property across different seasons and different temperatures of handled objects. The relative DNA amounts obtained from repeatedly handled tubes, worn T-shirts, and from faces reflected the shedder status of the participants. We suggest that an individual’s shedder status is highly influenced by the DNA levels on other body parts than hands, accumulating on the palms by frequently touching e.g., the face or previously handled items harboring self-DNA. Assessing physiological differences between the participants revealed that there were no associations between DNA shedding and individual sweat rates.

脱落状态被定义为个体将 DNA 留在接触过的物品或表面上的倾向，并被认为是影响 DNA 转移的主要因素之一。然而，对于脱落者状态是否是个体在多次测量中或环境条件变化时的恒定属性，人们知之甚少。我们对 6 名男性的 DNA 沉积物进行了 20 次评估，以获得参考数据集，并将参与者分为高、中或低脱落者。该数据集还用于研究当 DNA 沉积测量数量增加时，正确脱落状态分类的概率如何变化。使用 VapoMeter 测量个人出汗率，并通过每次采样的调查问卷收集有关卫生习惯的数据。接下来，我们研究了实验条件的变化（例如季节变化、卫生习惯、触摸物体的温度以及反复处理物体）如何影响 DNA 脱落。此外，我们还评估了从 6 名参与者的面部和 T 恤中收集的 DNA，以探讨脱落者状态是否与从身体其他部位获得的 DNA 相对量有关。我们的结果表明，脱落状态在不同季节和所处理物体的不同温度下是稳定的特性。从反复处理的试管、穿过的 T 恤和面部获得的相对 DNA 含量反映了参与者的脱落状态。我们认为，一个人的脱落状态很大程度上受到手以外其他身体部位的 DNA 水平的影响，通过经常触摸脸部或以前处理过的含有自身 DNA 的物品，DNA 水平会在手掌上积累。评估参与者之间的生理差异表明，DNA 脱落与个人出汗率之间没有关联。