Hematopoietic stem cells (HSCs) represent crucial target cells in the management of hematopoietic and immune system disorders. Unfortunately, the primary source of hematopoietic stem cells is limited. Hematopoietic stem cells derived from induced pluripotent stem cells (iPSCs) hold great promise for applications in cell therapy, disease modeling, and drug screening. To achieve a consistent induction method, one specific induction scheme capable of reliably generating CD34 and CD45 double-positive cells from iPSCs was optimized, employing a comparative analysis and screening of various induction methods. The comprehensive induction procedures are outlined in this document.

中文翻译：

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使用单层系统体外分化人 CD45+ CD34+ 诱导的 iPSC 造血祖细胞

造血干细胞（HSC）是治疗造血和免疫系统疾病的关键靶细胞。不幸的是，造血干细胞的主要来源是有限的。源自诱导多能干细胞 (iPSC) 的造血干细胞在细胞治疗、疾病建模和药物筛选方面具有广阔的应用前景。为了实现一致的诱导方法，通过对各种诱导方法的比较分析和筛选，优化了一种能够可靠地从 iPSC 产生 CD34 和 CD45 双阳性细胞的特定诱导方案。本文件概述了全面的入职程序。