It is well established that mouse ovarian granulosa cells secrete urokinase plasminogen activator (uPA) under gonadotropin stimulation. The synthesis and secretion of the enzyme correlate well with the time of follicular rupture in vivo. Moreover, uPA is secreted by the trophoblast at the time of implantation. In the present study, we have analyzed whether the absence of uPA could influence follicular growth, ovulation, and embryo implantation. Our data show fewer preantral follicles in ovaries but no decrease in hormonally induced ovulation. However, we observed a significant decrease in the number of implanted embryos in animals and, therefore, a lower number of pups per family. Adding uPA to the epithelial and stromal uterine cell culture medium strongly upregulates the expression of prostaglandin-endoperoxide synthase 2 ), the enzyme required for prostaglandin production and embryo implantation. The uPA inhibitor amiloride abrogated this increase.

中文翻译：

---

缺乏尿激酶纤溶酶原激活剂的雌性小鼠的生育能力下降

众所周知，小鼠卵巢颗粒细胞在促性腺激素刺激下分泌尿激酶纤溶酶原激活剂（uPA）。该酶的合成和分泌与体内卵泡破裂的时间密切相关。此外，uPA是在植入时由滋养层分泌的。在本研究中，我们分析了 uPA 的缺失是否会影响卵泡生长、排卵和胚胎着床。我们的数据显示，卵巢腔前卵泡减少，但激素诱导的排卵并未减少。然而，我们观察到动物体内植入的胚胎数量显着减少，因此每个家庭的幼崽数量也减少了。在上皮细胞和基质子宫细胞培养基中添加 uPA 会强烈上调前列腺素内过氧化物合酶 2 ) 的表达，前列腺素内过氧化物合酶是前列腺素产生和胚胎植入所需的酶。 uPA抑制剂阿米洛利消除了这种增加。