A protein-inspired ion-imprinted polymer (IIP) was used as an additive in a carbon paste electrode for the selective determination of copper(II) in water samples. The imprinted polymer was synthesized by precipitation polymerization of a functional monomer, methacrylamido-L-histidine (MAH), based on an amino acid, L-histidine, with ethylene glycol dimethacrylate (EGDMA) as crosslinker. The corresponding non-imprinted polymer (NIP) and a copolymer containing EGDMA and methyl methacrylate (MMA) (in the same proportions as MAH in the NIP) were also prepared in similar conditions as the IIP. The polymer particles were first chemically characterized by ¹³C CP-MAS NMR and FT-IR and the presence of MAH was confirmed by the colorimetric Pauly's test. It was shown by SEM and adsorption/desorption of nitrogen that the IIP and the NIP present similar morphological properties. The copper(II) binding properties of the polymers were also studied in a batch adsorption experiment, and an imprinting effect was observed for the IIP. This polymer was shown to possess binding cavities selective towards copper(II) versus common interferents (iron(III), zinc(II) and lead(II)), when compared to the NIP and to the poly(EGDMA-co-MMA). A carbon paste working electrode was prepared with the IIP particles and a limit of detection of 59 nmol/L for copper(II) was determined in a potassium phosphate buffer (0.1 M, pH = 7). The determination of copper(II) was not affected by the presence of a 50-fold excess of iron(III), zinc(II) and lead(II). Finally, the copper concentration in a real seawater was estimated to be 0.12 μmol/L, in full agreement with a measurement by polarography which was used as a reference method.

蛋白质启发的离子印迹聚合物 (IIP) 用作碳糊电极中的添加剂，用于选择性测定水样中的铜 (II)。印迹聚合物是通过以氨基酸L-组氨酸为基础的功能单体甲基丙烯酰氨基-L-组氨酸(MAH)与二甲基丙烯酸乙二醇酯(EGDMA)作为交联剂的沉淀聚合来合成的。相应的非印迹聚合物（NIP）和含有EGDMA和甲基丙烯酸甲酯（MMA）的共聚物（与NIP中MAH的比例相同）也在与IIP类似的条件下制备。聚合物颗粒首先通过¹³C CP-MAS NMR 和 FT-IR 进行化学表征，并通过比色保利测试证实了 MAH 的存在。SEM和氮气吸附/解吸表明IIP和NIP具有相似的形态特征。还在批量吸附实验中研究了聚合物的铜（II）结合特性，并观察到 ​​IIP 的印迹效应。与 NIP 和聚 (EGDMA-co-MMA) 相比，该聚合物对铜 (II) 和常见干扰物（铁 (III)、锌 (II) 和铅 (II)）具有选择性结合空腔）。使用 IIP 颗粒制备碳糊工作电极，并在磷酸钾缓冲液（0.1 M，pH = 7 ）中测定铜 (II) 的检测限为 59 nmol/L 。铜 (II) 的测定不受过量 50 倍的铁 (III)、锌 (II) 和铅 (II) 的影响。最终，真实海水中的铜浓度估计为0.12 μmol/L，与作为参考方法的极谱测量完全一致。