The failure of breeding strategies has caused scientists to shift to other means where the new approach involves exploring the microbiome to modulate plant defense mechanisms against Cotton Leaf Curl Disease (CLCuD). The cotton microbiome of CLCuD-resistant varieties may harbor a multitude of bacterial genera that significantly contribute to disease resistance and provide information on metabolic pathways that differ between the susceptible and resistant varieties. The current study explores the microbiome of CLCuD-susceptible Gossypium hirsutum and CLCuD-resistant Gossypium arboreum using 16 S rRNA gene amplification for the leaf endophyte, leaf epiphyte, rhizosphere, and root endophyte of the two cotton species. This revealed that Pseudomonas inhabited the rhizosphere while Bacillus was predominantly found in the phyllosphere of CLCuV-resistant G. arboreum. Using salicylic acid-producing Serratia spp. and Fictibacillus spp. isolated from CLCuD-resistant G. arboreum, and guided by our analyses, we have successfully suppressed CLCuD in the susceptible G. hirsutum through pot assays. The applied strains exhibited less than 10% CLCuD incidence as compared to control group where it was 40% at 40 days post viral inoculation. Through detailed analytics, we have successfully demonstrated that the applied microbes serve as a biocontrol agent to suppress viral disease in Cotton.

育种策略的失败导致科学家转向其他方法，其中新方法涉及探索微生物组来调节植物针对棉花卷叶病（CLCuD）的防御机制。CLCuD 抗性品种的棉花微生物组可能含有多种细菌属，这些细菌属对抗病性有显着贡献，并提供有关易感品种和抗性品种之间不同代谢途径的信息。目前的研究利用 16 S rRNA 基因扩增技术，对两种棉种的叶内生菌、叶附生菌、根际和根内生菌的CLCuD 敏感陆地棉和 CLCuD 抗性棉花的微生物组进行了探索。这表明假单胞菌栖息在根际，而芽孢杆菌主要存在于抗 CLCuV 的G. arboreum的叶际中。使用产水杨酸的沙雷氏菌属。和假芽孢杆菌属。从对 CLCuD 具有抗性的G. arboreum 中分离出来，并在我们的分析指导下，我们通过盆栽试验成功抑制了易感G. hirsutum中的 CLCuD 。与对照组相比，所应用的菌株表现出低于 10% 的 CLCuD 发病率，对照组在病毒接种后 40 天的发病率为 40%。通过详细的分析，我们成功证明了所应用的微生物可以作为抑制棉花病毒病的生物防治剂。