Patients negative for the JAK2 p.V617F somatic variant are frequently reflexed to testing for MPL exon 10 variants. Detection of these variants via multiplexed allele-specific PCR followed by fragment analysis has been previously published. The present study builds on this concept by improving the detection of the p.W515A variant, adding a second allele-specific primer to detect the p.W515R variant, and incorporating an improved primer for p.S505N detection.

中文翻译：

---

骨髓增生性肿瘤中 MPL 密码子 p.W515 和 p.S505N 突变的扩展分子检测

JAK2 p.V617F 体细胞变异呈阴性的患者经常会反射性地检测MPL外显子 10 变异。先前已发表通过多重等位基因特异性 PCR 检测这些变异，然后进行片段分析。本研究基于这一概念，改进了 p.W515A 变体的检测，添加了第二个等位基因特异性引物来检测 p.W515R 变体，并纳入了用于 p.S505N 检测的改进引物。