Genetic component assembly is key in the simulation and implementation of genetic circuits. Automating this process, thus accelerating prototyping, is a necessity. We present pyBrick-DNA, a software written in Python, that assembles components for the construction of genetic circuits. pyBrick-DNA (colab.pyBrick.com) is a user-friendly environment where scientists can select genetic sequences or input custom sequences to build genetic assemblies. All components are modularly fused to generate a ready-to-go single DNA fragment. It includes Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and plant gene-editing components. Hence, pyBrick-DNA can generate a functional CRISPR construct composed of a single-guided RNA integrated with Cas9, promoters, and terminator elements. The outcome is a DNA sequence, along with a graphical representation, composed of user-selected genetic parts, ready to be synthesized and cloned in vivo. Moreover, the sequence can be exported as a GenBank file allowing its use with other synthetic biology tools.

中文翻译：

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pyBrick-DNA：基于 Python 的自动遗传组件组装环境。

遗传组件组装是遗传电路模拟和实现的关键。必须使该过程自动化，从而加速原型设计。我们推出了 pyBrick-DNA，这是一种用 Python 编写的软件，可以组装用于构建遗传电路的组件。pyBrick-DNA (colab.pyBrick.com) 是一个用户友好的环境，科学家可以在其中选择基因序列或输入自定义序列来构建基因组件。所有组件均以模块化方式融合，生成现成的单个 DNA 片段。它包括成簇规则间隔短回文重复序列 (CRISPR) 和植物基因编辑组件。因此，pyBrick-DNA 可以生成功能性 CRISPR 构建体，该构建体由与 Cas9、启动子和终止子元件集成的单引导 RNA 组成。结果是 DNA 序列以及图形表示，由用户选择的遗传部分组成，准备在体内合成和克隆。此外，该序列可以导出为 GenBank 文件，以便与其他合成生物学工具一起使用。