The urinary bladder harbors a community of microbes termed the urobiome, which remains understudied. In this study, we present the urobiome of healthy infant males from samples collected by transurethral catheterization. Using a combination of enhanced culture and amplicon sequencing, we identify several common bacterial genera that can be further investigated for their effects on urinary health across the lifespan. Many genera were shared between all samples suggesting a consistent urobiome composition among this cohort. We note that, for this cohort, early life exposures including mode of birth (vaginal vs. Cesarean section), or prior antibiotic exposure did not influence urobiome composition. In addition, we report the isolation of culturable bacteria from the bladders of these infant males, including Actinotignum spp., a bacterial genus that has been associated with urinary tract infections in older male adults. Herein, we isolate and sequence 9 distinct strains of Actinotignum spp. enhancing the genomic knowledge surrounding this genus and opening avenues for delineating the microbiology of this urobiome constituent. Furthermore, we present a framework for using the combination of culture-dependent and sequencing methodologies for uncovering mechanisms in the urobiome.

膀胱中存在着一个被称为“尿生物组”的微生物群落，目前该微生物群落的研究尚未充分。在这项研究中，我们通过经尿道导管插入术收集的样本展示了健康男性婴儿的尿生物组。通过结合增强培养和扩增子测序，我们确定了几种常见的细菌属，可以进一步研究它们对整个生命周期泌尿系统健康的影响。所有样本之间共享许多属，表明该队列中具有一致的尿生物组组成。我们注意到，对于这个队列来说，早期接触包括出生方式（阴道分娩与剖腹产）或之前的抗生素接触不会影响尿生物组的组成。此外，我们报告从这些男性婴儿的膀胱中分离出可培养的细菌，包括Actinotignum spp.，这是一种与老年男性尿路感染有关的细菌属。在此，我们分离并测序了 9 个不同的Actinotignum spp菌株。增强围绕该属的基因组知识，并为描述该泌尿组成分的微生物学开辟途径。此外，我们提出了一个框架，结合使用培养依赖性和测序方法来揭示尿路生物组的机制。