Interactions between a zwitterionic phospholipid, 1, 2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) and four anionic phospholipids dihexadecyl phosphate (DHP), 1, 2-dimyristoyl-sn-glycero-3-phosphoglycerol (DMPG), 1, 2-dipalmitoyl-sn-glycero-3-phosphate (DPP) and 1, 2-dipalmitoyl-sn-glycero-3-phospho ethanol (DPPEth) in combination with an additional amount of 30 mol% cholesterol were separately investigated at air-buffer interface through surface pressure (π) - area (A) measurements. π-A isotherm derived parameters revealed maximum negative deviation from ideality for the mixtures comprising 30 mol% anionic lipids. Besides the film functionality, structural changes of the monomolecular films at different surface pressures in the absence and presence of polyamidoamine (PAMAM, generation 4), a cationic dendrimer, were visualised through Brewster angle microscopy and fluorescence microscopic studies. Fluidity/rigidity of monolayers were assessed by surface dilatational rheology studies. Effect of PAMAM on the formation of adsorbed monolayer, due to bilayer disintegration of liposomes (DPPC:anionic lipids= 7:3 M/M, and 30 mol% cholesterol) were monitored by surface pressure (π) - time (t) isotherms. Bilayer disintegration kinetics were dependent on lipid head group and chain length, besides dendrimer concentration. Such studies are considered to be an in vitro cell membrane model where the alteration of molecular orientation play important roles in understanding the nature of interaction between the dendrimer and cell membrane. Liposome-dendrimer aggregates were nontoxic to breast cancer cell line as well as in doxorubicin treated MDA-MB-468 cell line suggesting their potential as drug delivery systems.

两性离子磷脂 1, 2-二棕榈酰-sn-甘油-3-磷脂酰胆碱 (DPPC) 和四种阴离子磷脂磷酸二十六烷基酯 (DHP), 1, 2-二肉豆蔻酰-sn-甘油-3-磷酸甘油 (DMPG), 1 之间的相互作用, 2-二棕榈酰-sn-甘油-3-磷酸 (DPP) 和 1, 2-二棕榈酰-sn-甘油-3-磷酸乙醇 (DPPEth) 与额外量的 30 mol% 胆固醇在空气中分别进行研究缓冲界面通过表面压力（π）-面积（A）测量。 π-A 等温线导出的参数揭示了包含 30 mol% 阴离子脂质的混合物与理想状态的最大负偏差。除了薄膜功能之外，通过布鲁斯特角显微镜和荧光显微镜研究，还可以在不存在和存在聚酰胺胺（PAMAM，第 4 代）（一种阳离子树枝状聚合物）的情况下，在不同表面压力下观察单分子薄膜的结构变化。通过表面膨胀流变学研究评估单层的流动性/刚性。通过表面压力 (π) - 时间 (t) 等温线监测由于脂质体双层崩解（DPPC：阴离子脂质 = 7:3 M/M 和 30 mol% 胆固醇），PAMAM 对吸附单层形成的影响。除树枝状聚合物浓度外，双层崩解动力学还取决于脂质头基和链长。此类研究被认为是体外细胞膜模型，其中分子取向的改变在理解树枝状聚合物和细胞膜之间相互作用的性质方面发挥着重要作用。脂质体-树枝状聚合物聚集体对乳腺癌细胞系以及阿霉素处理的 MDA-MB-468 细胞系无毒，表明它们作为药物递送系统的潜力。