Due to the increasing demand for green technologies for the extraction of essential oils, natural deep eutectic solvents (NADES) have emerged as a type of environmentally friendly solvents. This work aimed to study the use of different NADES in combination with microwave pretreatment for intensifying the extraction of Aloysya citriodora (Mexican lippie) essential oil (ACEO), a barely studied plant. The chemical composition (GC-MS) and biological activities (antimicrobial activity, antioxidant activity, and total phenolic compounds) of the obtained oils are reported. The yield of ACEO extracted with NADES increased from 0.10 to 0.21%. The main compounds of each ACEO were verbenone (28-54%), limonene (2-10%), and spathulenol (3-15%). ACEOs extracted with NADES showed better antimicrobial activity against gram-positive bacteria. The ACEO extracted by Ch:OA reduced 3.6 log CFU/mL against Listeria monocytogenes. Meanwhile, ACEOs extracted by Ch:CA and Ch:OA showed the highest antioxidant activities (27.77-35.95 mg TE/mL of EO), and total phenolic compounds (15.33-17.35 mg GAE/mL of EO). Overall results suggest that using these green solvents aided the extraction method and may represent a study opportunity for extracting natural products without harming the environment, improving the process efficiency through a sustainable and innovative approach, with promising industrial applications.

由于对精油提取绿色技术的需求不断增加，天然低共熔溶剂（NADES）作为一种环保溶剂应运而生。这项工作旨在研究使用不同的 NADES 与微波预处理相结合来强化提取Aloysya citriodora（墨西哥 Lippie）精油（ACEO），这是一种很少被研究的植物。报告了所得油的化学成分 (GC-MS) 和生物活性（抗菌活性、抗氧化活性和总酚化合物）。用NADES提取的ACEO的产率从0.10%增加到0.21%。每种ACEO的主要化合物是马鞭草酮(28-54%)、柠檬烯(2-10%)和spathulenol(3-15%)。用 NADES 提取的 ACEO 对革兰氏阳性菌表现出更好的抗菌活性。Ch:OA 提取的 ACEO 对单核细胞增生李斯特氏菌的抑制作用为 3.6 log CFU/mL 。同时，Ch:CA和Ch:OA提取的ACEO表现出最高的抗氧化活性（27.77-35.95 mg TE/mL EO）和总酚化合物（15.33-17.35 mg GAE/mL EO）。总体结果表明，使用这些绿色溶剂有助于提取方法，并可能代表在不损害环境的情况下提取天然产物的研究机会，通过可持续和创新的方法提高工艺效率，具有广阔的工业应用前景。