Flavonoids are important secondary metabolites, but their separation and enrichment are difficult due to low contents and complex active components. Poly(styrene-divinylbenzene)-based resins are widely used for the separation of natural products, however, they have low adsorption capacity and poor selectivity. To solve these problems, Mannich reaction modified magnetic poly(styrene-divinylbenzene) (MPM-MR) microspheres were synthesized, and used for the separation and purification of flavonoids from Lycium barbarum leaves. Compared with other flavonoids (such as kaempferol, quercetin, naringenin and rutin), MPM-MR exhibited higher adsorption capacity and better selectivity for myricetin, which reached 81.6 mg/g at 298.15 K. Sieving interactions, hydrogen bonds and π-π interactions mainly exist between the MPM-MR and the flavonoids. In addition, the materials showed good enrichment of total flavonoids from actual samples of Lycium barbarum leaves, which increased from 8.17% to 24.99%. The Mannich reaction-modified magnetic poly(styrene-divinylbenzene) would be a promising material for efficient capture of flavonoids from natural plants.

黄酮类化合物是重要的次生代谢产物，但其含量低、活性成分复杂，分离富集困难。聚苯乙烯-二乙烯基苯树脂广泛用于天然产物的分离，但其吸附容量低、选择性差。针对这些问题，合成了曼尼希反应修饰的磁性聚苯乙烯-二乙烯基苯（MPM-MR）微球，并将其用于枸杞叶中黄酮类化合物的分离纯化。与其他黄酮类化合物（如山奈酚、槲皮素、柚皮素和芦丁）相比，MPM-MR 对杨梅素表现出更高的吸附容量和更好的选择性，在 298.15 K 时达到 81.6 mg/g。筛分相互作用、氢键和 π-π 相互作用主要存在于 MPM-MR 和黄酮类化合物之间。此外，该材料对枸杞叶实际样品中的总黄酮含量也表现出良好的富集作用，从8.17%提高到24.99%。曼尼希反应改性的磁性聚（苯乙烯-二乙烯基苯）将成为有效捕获天然植物中黄酮类化合物的有前途的材料。