Thus far, several monoclonal antibodies directed against cell-surface carbohydrate antigens have been generated. Among them, R-10G reportedly reacts selectively with human embryonic stem and induced pluripotent stem cells, but not with embryonal carcinoma (EC) cells. However, EC cells derived from patients' EC tumors may exhibit varying levels of R-10G-reactive antigen expression. Thus, we asked whether human EC tissues or germ cell tumor (GCT) tissues other than EC express R-10G-reactive antigen. To do so, we quantitatively analyzed R-10G-reactive antigen expression in 83 testicular GCT surgical specimens containing a total of 125 various GCT components. Accordingly, in all EC components examined, the EC cell plasma membrane was immunolabeled with R-10G, while most seminoma components were R-10G-negative. In non-seminomatous GCT (NSGCT) other than EC (non-EC NSGCT), R-10G-reactive antigen expression was variable, but signal distribution was focal, and the average intensity was weaker than that seen in EC. The percentages of R-10G-positive cells in these three groups varied with high statistical significance (p<0.001 for all combinations). These findings indicate that the R-10G-reactive antigen is preferentially expressed in human testicular EC tissues and, thus, could be used as a diagnostic marker for this malignancy.

中文翻译：

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R-10G 反应抗原在人睾丸胚胎癌中的矛盾表达。

迄今为止，已经产生了几种针对细胞表面碳水化合物抗原的单克隆抗体。其中，据报道，R-10G 选择性地与人胚胎干细胞和诱导多能干细胞发生反应，但不与胚胎癌细胞 (EC) 发生反应。然而，源自患者 EC 肿瘤的 EC 细胞可能表现出不同水平的 R-10G 反应性抗原表达。因此，我们询问人EC组织或EC以外的生殖细胞肿瘤（GCT）组织是否表达R-10G反应抗原。为此，我们定量分析了 83 个睾丸 GCT 手术标本中的 R-10G 反应抗原表达，其中总共包含 125 种不同的 GCT 成分。因此，在所有检查的 EC 成分中，EC 细胞质膜均用 R-10G 进行免疫标记，而大多数精原细胞瘤成分为 R-10G 阴性。在除EC（non-EC NSGCT）之外的非精原细胞GCT（NSGCT）中，R-10G反应性抗原表达变化，但信号分布集中，平均强度弱于EC。这三组中 R-10G 阳性细胞的百分比变化具有高度统计显着性（所有组合的 p<0.001）。这些发现表明，R-10G 反应性抗原优先在人睾丸 EC 组织中表达，因此可以用作这种恶性肿瘤的诊断标记物。