Scientific Reports ( IF 3.8 ) Pub Date : 2023-11-14 , DOI: 10.1038/s41598-023-46879-2 Koray Ergunay 1, 2, 3, 4 , Brian P Bourke 1, 2, 3 , Drew D Reinbold-Wasson 5 , Mikeljon P Nikolich 6 , Suppaluck P Nelson 1, 2, 3 , Laura Caicedo-Quiroga 1, 2, 3 , Nataliya Vaydayko 7 , Giorgi Kirkitadze 5 , Tamar Chunashvili 5 , Lewis S Long 8 , Jason K Blackburn 9 , Nora G Cleary 10 , Cynthia L Tucker 1, 2 , Yvonne-Marie Linton 1, 2, 3
We analysed both pooled and individual tick samples collected from four countries in Eastern Europe and the Black Sea region, using metagenome-based nanopore sequencing (NS) and targeted amplification. Initially, 1337 ticks, belonging to 11 species, were screened in 217 pools. Viruses (21 taxa) and human pathogens were detected in 46.5% and 7.3%, respectively. Tick-borne viral pathogens comprised Tacheng Tick Virus 2 (TTV2, 5.9%), Jingmen Tick Virus (JMTV, 0.9%) and Tacheng Tick Virus 1 (TTV1, 0.4%). An association of tick species with individual virus taxa was observed, with the exception of TTV2, which was observed in both Dermacentor and Haemaphysalis species. Individual ticks from pools with pathogen detection were then further screened by targeted amplification and then NS, which provided extensive genome data and revealed probable pathogen Haseki Tick Virus (HTV, 10.2%). Two distinct TTV2 clades were observed in phylogenetic analysis, one of which included closely related Dermacentor reticulatus Uukuviruses. JMTV detection indicated integrated virus sequences. Overall, we observed an expansion of newly documented pathogenic tick-borne viruses into Europe, with TTV1 being identified on the continent for the first time. These viruses should be included in the diagnostic assessment of symptomatic cases associated with tick bites and vector surveillance efforts. NS is shown as a useful tool for monitoring tick-associated pathogens in pooled or individual samples.
中文翻译:
东欧和黑海地区新出现的蜱传病毒范围不断扩大
我们使用基于宏基因组的纳米孔测序 (NS) 和靶向扩增,分析了从东欧和黑海地区四个国家收集的混合蜱样本和个体蜱样本。最初,在 217 个池中筛查了属于 11 个物种的 1337 只蜱虫。病毒(21 个分类群)和人类病原体的检出率分别为 46.5% 和 7.3%。蜱传病毒病原体包括塔城蜱病毒2型(TTV2,5.9%)、荆门蜱病毒(JMTV,0.9%)和塔城蜱病毒1型(TTV1,0.4%)。观察到蜱种类与单个病毒类群之间存在关联,但 TTV2 除外,在Dermacentor和Haemaphysalis种类中都观察到了 TTV2。然后通过靶向扩增和 NS 进一步筛选经过病原体检测的池中的个体蜱,这提供了广泛的基因组数据并揭示了可能的病原体长谷蜱病毒(HTV,10.2%)。在系统发育分析中观察到两个不同的 TTV2 进化枝,其中之一包括密切相关的网状革皮病毒Uukuviruses。JMTV检测显示整合的病毒序列。总体而言,我们观察到新记录的致病性蜱传病毒已扩展到欧洲,其中 TTV1 首次在欧洲大陆被发现。这些病毒应纳入与蜱虫叮咬相关的症状病例的诊断评估和媒介监测工作中。NS 被证明是监测混合样本或个体样本中蜱相关病原体的有用工具。