In this study, we investigated the microencapsulation of two strains of the entomopathogenic bacteria Bacillus thuringiensis (B. thuringiensis) (BtMA-750 and BtMA-1114), which are biopesticides of high toxicity for the mosquito vector Aedes aegypti. The encapsulation of different concentrations of microorganisms in starch microparticles was evaluated, and the inverse suspension polymerization technique was explored. It was possible to observe that the higher amounts of the biopesticide caused a slight decrease in the diameter of the particles; however, even when encapsulated, the biopesticide still presents an average diameter that is able to be consumed by the larvae of Aedes aegypti. Furthermore, it was noticed that the presence of both of the B. thuringiensis strains did not affect the thermal stability of the particles. The microencapsulated bacterial strains presented a high number of viable spores and preserved the expression of proteins with molecular masses corresponding to the insecticidal toxins Cry and Cyt, indicating that the encapsulation process was conducted satisfactorily. Finally, the encapsulated strains were tested against Ae. aegypti larvae and maintained 100% larval mortality even after 35 days. Therefore, microencapsulation of B. thuringiensis not only guarantees the bacterial activity, but also prolongs the action of the biopesticide. Collectively, such findings highlight the great potential of the new biopesticides, which may help to reduce the population indices of the mosquito vector Ae. aegypti via a sustainable and environment-friendly route.

在本研究中，我们研究了两种昆虫病原菌苏云金芽孢杆菌（B. thuringiensis）（BtMA-750和BtMA-1114）的微囊化，它们是对蚊媒埃及伊蚊具有高毒性的生物农药。评价了不同浓度微生物在淀粉微粒中的包封情况，并探索了反相悬浮聚合技术。可以观察到较高含量的生物农药导致颗粒直径略有减小；然而，即使被封装，生物农药仍然具有能够被埃及伊蚊幼虫消耗的平均直径。此外，注意到两种苏云金芽孢杆菌菌株的存在并不影响颗粒的热稳定性。微囊化菌株呈现出大量的活孢子，并保留了与杀虫毒素Cry和Cyt相对应的分子量的蛋白质的表达，表明封装过程令人满意。最后，对封装的菌株进行抗伊蚊测试。埃及伊蚊幼虫，即使在 35 天后仍保持 100% 的幼虫死亡率。因此，将苏云金芽孢杆菌微胶囊化不仅保证了细菌活性，而且延长了生物农药的作用。总的来说，这些发现凸显了新型生物农药的巨大潜力，可能有助于减少蚊媒伊蚊的种群指数。埃及伊蚊通过可持续且环境友好的路线。