This article provides an extensive review of the advancements and future perspectives related to endoscopic ultrasound-guided tissue acquisition (EUS-TA) for the diagnosis of solid pancreatic lesions (SPLs). EUS-TA, including fine-needle aspiration (EUS-FNA) and fine-needle biopsy (EUS-FNB), has revolutionized the collection of specimens from intra-abdominal organs, including the pancreas. Improvements in the design of needles, collection methods, and specimen processing techniques have improved the diagnostic performance. This review highlights the latest findings regarding needle evolution, actuation number, sampling methods, specimen evaluation techniques, application of artificial intelligence (AI) for diagnostic purposes, and use of comprehensive genomic profiling (CGP). It acknowledges the rising use of Franseen and fork-tip needles for EUS-FNB and emphasizes that the optimal number of actuations requires further study. Methods such as the door-knocking and fanning techniques have shown promise for increasing diagnostic performance. Macroscopic on-site evaluation (MOSE) is presented as a practical rapid specimen evaluation method, and the integration of AI is identified as a potentially impactful development. The study also underscores the importance of optimal sampling for CGP, which can enhance the precision of cancer treatment. Ongoing research and technological innovations will further improve the accuracy and efficacy of EUS-TA.

本文对用于诊断实性胰腺病变 (SPL) 的内镜超声引导组织采集 (EUS-TA) 相关的进展和未来前景进行了广泛的回顾。EUS-TA，包括细针抽吸 (EUS-FNA) 和细针活检 (EUS-FNB)，彻底改变了腹内器官（包括胰腺）样本的采集。针头设计、采集方法和标本处理技术的改进提高了诊断性能。本综述重点介绍了有关针进化、驱动次数、采样方法、样本评估技术、人工智能 (AI) 在诊断中的应用以及综合基因组分析 (CGP) 的使用的最新发现。它承认 Franseen 针和叉尖针在 EUS-FNB 中的使用不断增加，并强调最佳驱动次数需要进一步研究。敲门和扇扇技术等方法已显示出提高诊断性能的希望。宏观现场评估（MOSE）被认为是一种实用的快速样本评估方法，人工智能的集成被认为是一种潜在的有影响力的发展。该研究还强调了 CGP 最佳采样的重要性，它可以提高癌症治疗的精确度。持续的研究和技术创新将进一步提高 EUS-TA 的准确性和有效性。