Microbiological diagnosis of osteoarticular infections (OI) is crucial for a successful treatment. A prospective multicenter study including 262 synovial fluids with suspicion of acute OI was performed between July 2021 and October of 2022. BioFire Joint Infection Panel multiplex-PCR test was performed and results were compared with conventional cultures of synovial fluid specimens. In total, 136 microorganisms were detected, and fourteen samples were positive for more than one microorganism. In monomicrobial infections (n = 87) agreement with culture was 69%. In 26 samples, the multiplex PCR yield an additional positive result when culture result was negative. It helped in the detection of fastidious microorganisms as K. kingae and N. gonorrhoeae. This multiplex PCR has proven to be a useful technique that can be used for patients with high suspicion of acute OI in a rapid and automated manner.

骨关节感染 (OI) 的微生物学诊断对于成功治疗至关重要。2021 年 7 月至 2022 年 10 月期间进行了一项前瞻性多中心研究，纳入了 262 份疑似急性成骨不全的滑液。进行了 BioFire 联合感染小组多重 PCR 测试，并将结果与​​滑液标本的常规培养结果进行了比较。总共检测出 136 种微生物，其中 14 个样本对一种以上微生物呈阳性。在单一微生物感染 (n = 87) 中，培养结果的一致性为 69%。在 26 个样本中，当培养结果呈阴性时，多重 PCR 产生额外的阳性结果。它有助于检测金氏克雷伯菌和淋病奈瑟菌等挑剔的微生物。这种多重 PCR 已被证明是一种有用的技术，可以快速、自动化地用于高度怀疑急性 OI 的患者。