The fluorescence viewing of mitochondria is commonly performed by MitoTracker, a lipophilic cationic dye that is taken up by the mitochondria. In this forum, we highlight several issues that may occur with MitoTracker, including staining of other organelles. Our aim is to offer alternative dyes and discuss their advantages and disadvantages. We also offer options for software with alternatives to MitoTracker to expedite future experimental design.

中文翻译：

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MitoTracker：一个有用的工具，需要更好的替代品

线粒体的荧光观察通常由 MitoTracker 进行，MitoTracker 是一种被线粒体吸收的亲脂性阳离子染料。在本论坛中，我们重点介绍 MitoTracker 可能出现的几个问题，包括其他细胞器的染色。我们的目标是提供替代染料并讨论它们的优点和缺点。我们还提供 MitoTracker 替代品的软件选项，以加快未来的实验设计。