Single nucleotide polymorphism (SNP) genotyping technologies can generate investigative leads for human remains identification, including estimation of biological sex, biogeographical ancestry (BGA), externally visible characteristics (EVCs), identity, uniparental lineage and extended kinship. The ForenSeq® Kintelligence Kit provides forensic laboratories with the ability to apply this suite of genetic tools to forensic samples using one panel targeting 10,230 SNPs (including 56 ancestry-informative, 24 phenotype-informative, 94 identity-informative, 106 X chromosome, 85 Y chromosome and 9867 kinship-informative SNPs) sequenced on the MiSeq FGx® Sequencing System. The ForenSeq® Kintelligence Kit has been internally validated, optimised and operationalised by the Australian Federal Police National DNA Program for Unidentified and Missing Persons (AFP Program) for coronial casework. The internal validation was conducted according to the Scientific Working Group on DNA Analysis Methods guidelines (excluding mixture analysis), focussing on sample types typically encountered in human remains identification casework, such as bones, teeth, nail, blood and hair. The workflow was optimised for a high throughput library preparation and sequencing workflow, and additional analytical thresholds were developed to improve genotyping accuracy for low DNA input samples. Additionally, the genetic intelligence generated from the kit was compared to the self-declared biological sex, EVCs and BGA of the DNA donors to assess concordance. The kit was able to produce high quality SNP profiles from 1.0 ng down to 0.1 ng of DNA, with high repeatability and reproducibility, and minimal background noise. The prediction accuracy for biological sex (95%), hair colour (58%), eye colour (74%) and BGA inferences (consistent: 74%; partially consistent: 10%; inconclusive: 16%) was determined based on self-declared data. Additionally, SNP profiles from a volunteer family group of ten related individuals were uploaded to GEDmatch PRO™ to assess kinship accuracy. The kit was capable of detecting (97%) and accurately classifying (90%) genetic relationships spanning from first to fifth degree. The Kintelligence Kit provides the AFP Program with a robust and reliable genetic intelligence tool for unidentified and missing persons investigations, which has been designed to sequence multiple challenging samples in a single multiplexed assay using existing laboratory instrumentation.

单核苷酸多态性 (SNP) 基因分型技术可以为人类遗骸鉴定提供调查线索，包括生物性别、生物地理祖先 (BGA)、外部可见特征 (EVC)、身份、单亲血统和延伸亲属关系的估计。ForenSeq® Kintelligence 试剂盒使法医实验室能够使用针对 10,230 个 SNP（包括 56 个祖先信息、24 个表型信息、94 个身份信息、106 个 X 染色体、85 个 Y 染色体）的一个 panel，将这套遗传工具应用于法医样本。染色体和 9867 个亲属关系信息 SNP）在 MiSeq FGx® 测序系统上进行测序。ForenSeq® Kintelligence 试剂盒已由澳大利亚联邦警察国家身份不明者和失踪人员 DNA 计划（AFP 计划）针对验尸案件进行了内部验证、优化和操作。内部验证是根据 DNA 分析方法科学工作组指南（不包括混合物分析）进行的，重点关注人体遗骸鉴定案例中常见的样本类型，例如骨骼、牙齿、指甲、血液和头发。该工作流程针对高通量文库制备和测序工作流程进行了优化，并开发了额外的分析阈值以提高低 DNA 输入样本的基因分型准确性。此外，还将该试剂盒生成的遗传智能与 DNA 捐献者自我声明的生物性别、EVC 和 BGA 进行比较，以评估一致性。该试剂盒能够生成从 1.0 ng 到 0.1 ng DNA 的高质量 SNP 图谱，具有高重复性和再现性，以及最小的背景噪音。生物性别（95%）、头发颜色（58%）、眼睛颜色（74%）和 BGA 推断（一致：74%；部分一致：10%；不确定：16%）的预测准确度是根据自我评估确定的。声明的数据。此外，由 10 名相关人员组成的志愿者家庭组的 SNP 档案已上传至 GEDmatch PRO™，以评估亲属关系的准确性。该试剂盒能够检测 (97%) 并准确分类 (90%) 从一级到五级的遗传关系。Kintelligence 试剂盒为法新社项目提供了强大且可靠的遗传情报工具，用于身份不明和失踪人员调查，该工具旨在使用现有实验室仪器在单一多重检测中对多个具有挑战性的样本进行测序。