Targeted next-generation sequencing (NGS) is a powerful and suitable approach to comprehensively identify multiple types of variants in tumors. RNA-based NGS is increasingly playing an important role in precision oncology. Both parallel and sequential DNA- and RNA-based approaches are expensive, burdensome, and have long turnaround times, which can be impractical in clinical practice. A streamlined, unified DNA- and RNA-based NGS approach is urgently needed in clinical practice.

中文翻译：

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基于 DNA 和 RNA 的统一 NGS 策略，用于在实体瘤的双核酸水平上分析多种类型的变异

靶向下一代测序（NGS）是一种强大而合适的方法，可以全面识别肿瘤中的多种类型的变异。基于 RNA 的 NGS 在精准肿瘤学中发挥着越来越重要的作用。基于 DNA 和 RNA 的平行和连续方法均昂贵、繁重，并且周转时间长，这在临床实践中可能不切实际。临床实践中迫切需要一种简化、统一的基于 DNA 和 RNA 的 NGS 方法。