The identification of novel secreted factors is advancing at an unprecedented pace. However, there is a critical need to consolidate and integrate this knowledge to provide a framework of their diverse mechanisms, functional significance, and inter-relationships. Complicating this effort are challenges related to nonstandardized methods, discrepancies in sample handling, and inconsistencies in the annotation of unknown molecules. This Review aims to synthesize the rapidly expanding field of the metabolic secretome, encompassing the five major types of secreted factors: proteins, peptides, metabolites, lipids, and extracellular vesicles. By systematically defining the functions and detection of the components within the metabolic secretome, this Review provides a primer into the advances of the field, and how integration of the techniques discussed can provide a deeper understanding of the mechanisms underlying metabolic homeostasis and its disorders.

新型分泌因子的鉴定正在以前所未有的速度推进。然而，迫切需要巩固和整合这些知识，以提供其不同机制、功能意义和相互关系的框架。与非标准化方法、样品处理差异以及未知分子注释不一致相关的挑战使这项工作变得复杂。本综述旨在综合快速扩展的代谢分泌组领域，涵盖五种主要类型的分泌因子：蛋白质、肽、代谢物、脂质和细胞外囊泡。通过系统地定义代谢分泌组内各成分的功能和检测，这篇综述为该领域的进展提供了入门知识，以及所讨论的技术的整合如何能够更深入地了解代谢稳态及其紊乱的机制。