If a DNA sample collected in the field is old or degraded, short tandem repeat analysis is difficult to perform, a representative analysis method currently used for individual identification. Given that microorganisms exist everywhere and within the human body, in similar amounts to human cells, microbial analysis could be used to identify individuals even in cases in which human DNA-based identification is difficult. Research has demonstrated that the types of microorganisms within the human body differ depending on various internal or external factors, such as body part or bodily fluid type, lifestyle, geographical area of residence, sex, and age. In this study, we aimed to examine the relationship between lifestyle factors and the composition and diversity of the oral microbiome in individuals living in Korea. We collected 43 saliva samples from Korean individuals and analyzed the oral microbiome and its variations due to external factors, such as coffee consumption, drinking, and smoking. Linear discriminant analysis effect size revealed that Oribacterium, Campylobacter, and Megasphaera were abundant in coffee consumers, whereas Saccharimonadales, Clostridia, and Catonella were abundant in alcohol non-drinkers. We found increased levels of Stomatobaculum in the saliva of smokers, compared with that of non-smokers. Thus, our analysis revealed characteristic microorganisms for each parameter that was evaluated (coffee consumption, smoking, drinking). Consequently, our study provides insight into the oral microbiome in the Korean population and lays the foundation for developing the Korean Forensic Microbiome Database.

如果现场采集的 DNA 样本陈旧或降解，则很难进行短串联重复分析（目前用于个体识别的代表性分析方法）。鉴于微生物在人体内无处不在，其数量与人体细胞相似，即使在基于人类 DNA 的识别很困难的情况下，微生物分析也可用于识别个体。研究表明，人体内微生物的类型因各种内部或外部因素而异，例如身体部位或体液类型、生活方式、居住地理区域、性别和年龄。在这项研究中，我们旨在研究生活在韩国的个体的生活方式因素与口腔微生物组我们收集了 43 份韩国人的唾液样本，并分析了口腔微生物组及其因外部因素（例如咖啡摄入、饮酒和吸烟）引起的变化。线性判别分析效应大小显示，奥里杆菌属、弯曲杆菌属和巨球菌属在咖啡消费者中大量存在，而糖单胞菌属、梭菌属和卡东氏菌属在不饮酒者中大量存在。我们发现，与不吸烟者相比，吸烟者唾液中口腔杆菌因此，我们的分析揭示了每个评估参数（咖啡消费、吸烟、饮酒）的特征微生物。因此，我们的研究提供了对韩国人群口腔微生物组的深入了解，并为开发韩国法医微生物组数据库奠定了基础。