ABSTRACT

In this experimental study, adult female NMRI mice were randomly assigned to five groups: control ;(fresh ovarian transplantation, OT); sham ;(vitrified OT); NAC ;(vitrified OT treated with N-acetyl cysteine, NAC); EMF ;(vitrified OT treated with pulsed electromagnetic fields, PEMF); and NAC+EMF ;(vitrified OT combined with NAC and PEMF). We conducted histological assessments to evaluate follicle reservation and vascularization. Furthermore, we examined the relative expression of Fgf-2, Vegf, Tnf-α, Il-6, Il-1, and Cd31 genes on days 2 and 7 after OT. Additionally, we measured total antioxidant capacity (TAC), malondialdehyde (MDA) levels, as well as the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX). Our results demonstrated that NAC, PEMF, and NAC+PEMF treatments significantly increased the number of follicles. Moreover, we observed a more pronounced development of vascularization in the NAC, PEMF, and PEMF+NAC groups. The relative expression levels of Fgf-2, Vegf, Tnf-α, Il-1β, and Il-6 were significantly elevated in the NAC, PEMF, and NAC+PEMF groups. Notably, TAC levels decreased significantly in the NAC group compared to the control group. Additionally, the MDA level showed a significant decrease in the PEMF+NAC group when compared to the other groups. Overall, the combination of NAC and PEMF exhibited a synergistic effect in promoting angiogenesis and protecting against oxidative stress and inflammation during OT.

摘要

在这项实验研究中，成年雌性 NMRI 小鼠被随机分为五组：对照组（新鲜卵巢移植，OT）；假手术；（玻璃化 OT）；NAC ；（用 N-乙酰半胱氨酸处理的玻璃化 OT，NAC）；EMF；（用脉冲电磁场​​处理的玻璃化 OT，PEMF）；和NAC+EMF；（玻璃化OT与NAC和PEMF结合）。我们进行了组织学评估以评估卵泡保留和血管化。此外，我们检查了OT 后第 2 天和第 7 天Fgf-2、Vegf、Tnf-α、Il-6、Il-1和Cd31基因的相对表达。此外，我们还测量了总抗氧化能力 (TAC)、丙二醛 (MDA) 水平以及超氧化物歧化酶 (SOD) 和谷胱甘肽过氧化物酶 (GPX) 的活性。我们的结果表明，NAC、PEMF 和 NAC+PEMF 治疗显着增加了卵泡数量。此外，我们观察到 NAC、PEMF 和 PEMF+NAC 组的血管化发展更为明显。NAC、PEMF和NAC+PEMF组中Fgf-2、Vegf、Tnf-α、Il-1β和Il-6的相对表达水平显着升高。值得注意的是，与对照组相比，NAC 组的 TAC 水平显着下降。此外，与其他组相比，PEMF+NAC 组的 MDA 水平显着降低。总体而言，NAC 和 PEMF 的组合在促进血管生成和防止 OT 期间的氧化应激和炎症方面表现出协同作用。