Amphibians represent a useful taxon to study the evolution of sex determination because of their highly variable sex-determination systems. However, the sex-determination system for many amphibian families remains unknown, in part because of a lack of genomic resources. Here, using an F1 family of Green-eyed Treefrogs (Litoria serrata), we produce the first genetic linkage map for any Australo-Papuan Treefrogs (family: Pelodryadidae). The resulting linkage map contains 8662 SNPs across 13 linkage groups. Using an independent set of sexed adults, we identify a small region in linkage group 6 matching an XY sex-determination system. These results suggest Litoria serrata possesses a male heterogametic system, with a candidate sex-determination locus on linkage group 6. Furthermore, this linkage map represents the first genomic resource for Australo-Papuan Treefrogs, an ecologically diverse family of over 220 species.

两栖动物因其高度可变的性别决定系统而成为研究性别决定进化的有用分类单元。然而，许多两栖动物科的性别决定系统仍然未知，部分原因是缺乏基因组资源。在这里，我们利用绿眼树蛙 ( Litoria serrata ) 的 F1 家族，绘制了第一个澳大利亚-巴布亚树蛙（科：Pelodryadidae）的遗传连锁图谱。所得连锁图谱包含 13 个连锁群的 8662 个 SNP。使用一组独立的性别确定的成年人，我们在连锁群 6 中识别出一个与 XY 性别决定系统相匹配的小区域。这些结果表明，Litoria serrata拥有雄性异配系统，候选性别决定位点位于连锁群 6 上。此外，该连锁图代表了澳大利亚-巴布亚树蛙（一个包含 220 多个物种的生态多样性家族）的第一个基因组资源。