Abstract

Exercise-induced proteinuria has been widely investigated in humans, also in relation to intensity and duration of activity. Instead, there are only limited publications regarding urinary biochemical parameters and urinary proteins before and after physical activity in dogs. This paper aimed to investigate the effects of exercise on urinary biochemistry and proteins in military dogs. Twenty-four dogs were enrolled in this study. All the dogs were clinically sound, and they were examined before and after activity. Pulse rates (PR) and respiratory rate (RR) were monitored. Urine was sampled before and after a training session of search activity. Standard urinalysis was carried out, urine total proteins and creatinine were measured and the urinary protein:creatinine ratio was calculated; finally, the urinary proteins were separated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Clinical examination before and after activity did not reveal any pathological finding. After activity, the PR was slightly increased, while the RR was notably increased (p < 0.05). Total proteins, albumin, and their ratio with creatinine were significantly higher after exercise when considering all the dogs included or only the females while, when considering only the males no significant difference was detected. The clinical relevance of this study was related to the possibility of using urine as a non-invasive sample for monitoring health status after training activity and exercise in dogs. An increase in microalbuminuria after search activity, measured using SDS-PAGE could be considered an early biomarker of renal function during training sessions.

摘要

运动引起的蛋白尿已在人类中得到广泛研究，也与活动强度和持续时间有关。相反，关于狗体力活动前后的尿液生化参数和尿蛋白的出版物非常有限。本文旨在研究运动对军犬尿液生化和蛋白质的影响。二十四只狗参加了这项研究。所有狗的临床状况均良好，并且在活动前后均进行了检查。监测脉搏率（PR）和呼吸率（RR）。在搜索活动训练之前和之后采集尿液样本。进行标准尿液分析，测定尿总蛋白和肌酐，并计算尿蛋白：肌酐比值；最后，使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离尿蛋白。活动前后的临床检查未发现任何病理发现。活动后，PR 略有增加，而 RR 显着增加（p  < 0.05）。当考虑所有狗或仅考虑雌性时，运动后总蛋白、白蛋白及其与肌酐的比率显着升高，而当仅考虑雄性时，没有检测到显着差异。这项研究的临床意义与使用尿液作为非侵入性样本来监测狗训练活动和运动后健康状况的可能性有关。使用 SDS-PAGE 测量的搜索活动后微量白蛋白尿的增加可被视为训练期间肾功能的早期生物标志物。