We developed a curriculum for an upper-level molecular biology course-based undergraduate research laboratory class funded by a National Science Foundation CAREER grant that focuses on identifying new small proteins in the bacterium, Escherichia coli. Our CURE class has been continually offered each semester for the last 10 years, with multiple instructors collaboratively developing and implementing their own pedagogical approach while maintaining the same overall scientific goal and experimental strategy. In this paper, we delineate the experimental strategy for our molecular biology CURE laboratory class, describe a range of pedagogical approaches implemented by multiple instructors, and provide recommendations for teaching the class. The purpose of our paper is to share our experiences both in developing and teaching a molecular biology CURE laboratory class based on small protein identification and in creating a curriculum and support system that allows traditional, non-traditional, and under-represented students to participate in authentic research projects.

中文翻译：

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通过基于分子生物学课程的本科生研究经验实验室课程识别新的小蛋白质

我们为基于高级分子生物学课程的本科生研究实验室课程开发了课程，该课程由国家科学基金会职业补助金资助，重点是识别大肠杆菌中的新小蛋白质。在过去 10 年里，我们的 CURE 课程每学期都持续开设，多名教师共同开发和实施自己的教学方法，同时保持相同的总体科学目标和实验策略。在本文中，我们描述了分子生物学 CURE 实验室课程的实验策略，描述了由多名教师实施的一系列教学方法，并为课程教学提供了建议。我们论文的目的是分享我们在开发和教授基于小蛋白质鉴定的分子生物学 CURE 实验室课程以及创建课程和支持系统方面的经验，该课程和支持系统允许传统、非传统和代表性不足的学生参与真实的研究项目。